Dietary Methionine and Cysteine Restriction in Healthy Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Body weight, plasma sulfur amino acids
研究概览
简要总结
Studies in laboratory models indicate that dietary methionine restriction (MR) enhances longevity and produces short- and long-term metabolic changes that are consistent with a healthy lifespan. The present study was designed to establish levels of dietary methionine or methionine and cysteine that induce physiological changes in healthy adults like those observed in rodent studies .
详细描述
In this controlled feeding study, subjects will be randomized to one of two experimental. Group A (N=10): will start with control diet (31 mg/kg/day Met; 31 mg/kg/day Cys) for 4 weeks followed by a washout for 3-4 weeks, then a 70% MR diet (9.3 mg/kg/day Met; 40.3 mg/kg/day SAA) for 4 weeks, followed by 3-4 weeks washout period and then a 90% MR diet (3.1 mg/kg/day Met; 34.1 mg/kg/day SAA) for 4 weeks. Group B (N=10): will start with control diet (31 mg/kg/day Met; 31 mg/kg/day Cys) for 4 weeks followed by a washout for 3-4 weeks then a 50% methionine and cysteine restricted (M/CR) diet (15.5 mg/kg/day Met; 15.5 mg/kg/day Cys; 31 mg/kg/day SAA), followed by 3-4 weeks washout period and then a 65% M/CR diet (10.8 mg/kg/day Met; 10.8 mg/kg/day Cys; 21.6 mg/kg/day SAA) for 4 weeks.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 24 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy English speaking male and female volunteers
- •May or may not be students
- •Age range 24-65 years
- •Subjects will need to be of normal weight or moderately overweight (BMI 18.5-35 kg/m2)
- •Subjects will be non-smokers
排除标准
- •No use of medications known to impact the biomarkers of interest
- •Pregnancy and lactating women
- •Persons with documented diabetes
- •No use of high dose dietary antioxidant supplements or fish oil/flax seed oil for n-acetylcysteine for at least 1 month prior to the study
- •Use of tobacco products over the past 6 months
- •Unstable weight
- •Individuals with allergies to eggs, wheat, nuts, soy and latex
- •Individuals with phenylketonuria (PKU)
结局指标
主要结局
Body weight, plasma sulfur amino acids
时间窗: Baseline, four weeks
次要结局
- Biomarkers of oxidative stress, blood lipids(Baseline, four weeks)
研究者
John P. Richie
Professor of Public Health Sciences & Pharmacology
Milton S. Hershey Medical Center