The Effects of Mannose Supplementation on Glycosylation and Protein Levels in Human Sera
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 6
- 试验地点
- 2
- 主要终点
- Changes to glycan structure
研究概览
简要总结
The purpose of our study is to investigate the effects of supplemental mannose on sera of humans who are healthy. We will perform proteomics studies to elucidate changes to glycan structure and protein level changes. We hypothesize that we may find that mannose supplies more properly glycosylated protein substrate and helps expedite protein synthesis, that excess mannose leads to alternative glycan structures which confer improved stability, or that there are new sites that become glycosylated after mannose supplementation.
详细描述
Subjects will be enrolled and a baseline blood sample taken. Subjects will take mannose supplements for 1 week (3-4 packets of a standard dose in water), then a follow up blood sample will be taken.
Subject blood will be analyzed by proteomic analysis for 1) change in glycan structure or 2) other protein based approaches to measure changes in levels of proteins.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •At least 18 years of age
- •No known medical conditions
- •Taking no medications or nutritional supplements
- •Agree to the study and provide informed consent
排除标准
- •Less than 18 years of age
- •Known medical diagnosis, including pregnancy
- •Presently taking medications and/or nutritional supplements
- •Do not provide consent
研究组 & 干预措施
Mannose supplementation
Mannose supplementation will be given and subjects will be studied at baseline and after treatment.
干预措施: Mannose (Dietary Supplement)
结局指标
主要结局
Changes to glycan structure
时间窗: ~1 month
Glycopeptide mass spectrometry will be applied to the major glycoproteins present in serum.
Change in glycoprotein levels
时间窗: ~1 month
Standard laboratory methods such as ELISA in either research or clinical laboratory settings (Roche Cobas or similar platform).
次要结局
未报告次要终点
研究者
Jeffrey SoRelle
Assistant Professor
University of Texas Southwestern Medical Center