Impact of Exercise Training on Skeletal Muscle Capillary Maturation During Angiogenesis in COPD Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 22
- 试验地点
- 1
- 主要终点
- Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
研究概览
简要总结
COPD patients experiment a peripheral muscle dysfunction which impact their exercise tolerance and health-related quality of life. The capillary to fiber interface is reduced and impact the exercise capacity of the patients. While the muscle capillary creation in response to exercise training is blunted in COPD patients, the maturation of the neo-capillaries could also be blunted and contribute to the impaired aniogionenesis in patients. Because the capillary maturation is a sensitive and dynamic process, only different modalities of exercise training and multiple time-points of measures would allow to capture this microvascular adaptation.
Aim of the study : Compare the muscle capillary maturation in response to training at 5 and 10 weeks, in sedentary healthy subject trained at the intensity of the ventilatory threshold (60-65% of VO2max), versus :- COPD patients trained at a similar intensity (60-65% of VO2max)- COPD patients trained at a similar absolute intensity (90% of VO2max).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Care Provider, Outcomes Assessor)
入排标准
- 年龄范围
- 40 Years 至 78 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •COPD patients :
- •FEV1<80%pred.
- •non exacerbation during the past 4 weeks
- •no oxygen therapy, -
- •Healthy subjects:
- •<150min/week of moderate-to-vigorous physical activity,
- •Voorrips score <9
排除标准
- •Unstable comorbidity
- •Long term oxygen therapy or ventilation
- •Rehabilitation program during the past 12 months
- •nutritional supplementation during the past 4 weeks
- •allopurinol or N-acetylcystein during the past 4 weeks
- •long corticosteroid treatment during the past 6 months
- •hyperreactivity to xylocain
- •anticoagulant or platelet aggregation inhibitors treatment
- •participation to an other research study-pregnancy or impossibility to provide informed consent, ...
研究组 & 干预措施
COPD Patients ~90% VO2max
COPD Patients ~90% VO2max
干预措施: Muscular biopsy (Procedure)
COPD patients ~60-65% VO2max
COPD patients ~60-65% VO2max
干预措施: Muscular biopsy (Procedure)
healthy volunteers
healthy volunteers
干预措施: Muscular biopsy (Procedure)
healthy volunteers
healthy volunteers
干预措施: 5-10 weeks sport training (Other)
结局指标
主要结局
Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
时间窗: 10 weeks
Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
时间窗: 5 weeks
次要结局
- Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps(10 weeks)
- quadriceps endurance(10 weeks)
- Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vitro(10 weeks)
- Effect of the serum of COPD patients and SHS on pericyte morphology and in vitro(10 weeks)
- Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vivo(10 weeks)
- quadriceps endurance(5 weeks)
- Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps(5 weeks)