Effect of Plasma Uric Acid on Insulin Sensitivity, Endothelial Function and Inflammation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 31
- 试验地点
- 1
- 主要终点
- Percent Increase in Insulin-stimulated Glucose Uptake
研究概览
简要总结
The purpose of this study is to learn more about what is the effect of uric acid on oxidative stress and on the way the body metabolizes sugar in obese people. Understanding this may lead to better diseases such as diabetes.
详细描述
It has been hypothesized that oxidative stress is involved in the pathogenesis of insulin resistance associated with obesity. Circulating uric acid (UA) is the body's major endogenous plasma antioxidant. Therefore, the investigators evaluated whether alterations in serum UA concentration affect: 1) systemic and skeletal muscle oxidative stress, 2) total antioxidant capacity, and 4) skeletal muscle insulin sensitivity during a hyperinsulinemic-euglycemic clamp.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •obese (BMI 30-45 kg/m²);
- •serum UA concentration either ≥5 mg/dL or ≤4.0 mg/dL (297 µmol/L)],
排除标准
- •renal dysfunction (serum creatinine >1.5 mg/dL);
- •pregnancy or lactating;
- •take urate-lowering agents, thiazide diuretics, prednisone or medications containing aspirin or other salicylates;
- •history of xanthinuria, type 2 diabetes or other significant organ system dysfunction;
- •have G6PD deficiency;
- •use hormone-replacement or oral-contraceptive therapy;
- •smoke tobacco;
- •anemic (Hb <10 g/dl)
研究组 & 干预措施
Obese subjects with high uric acid
Subjects with a body mass index = or > 30 kg/m² with high uric acid (>6 mg/dL)
Intervention: one single infusion of rasburicase (0.19 mg/kg FFM) infused over 30 min
干预措施: Rasburicase (Drug)
结局指标
主要结局
Percent Increase in Insulin-stimulated Glucose Uptake
时间窗: 12 hours after reducing uric acid
Uric acid will be reduced to 0 with a 30 minute infusion of a uricase (Elitek, Sanofi-Aventis). A hyperinsulinemic-euglycemic clamp procedure in conjunction with stable isotope glucose tracer infusion will be used to measure percent increase in insulin-stimulated glucose uptake in obese subjects with high uric acid before and after uric acid reduction.
次要结局
- The Effect of Reducing Uric Acid on Oxidative Status(12 hours after reducing uric acid)
- Baseline Carbonylated Protein Ratio(Before reducing uric acid)
- AFTER Rasburicase Plasma TRAP(12 hours after reducing uric acid)
- Baseline Plasma FRAP(Before reducing uric acid)
- AFTER Rasburicase Carbonylated Protein Ratio(12 hours after reducing uric acid)
- Baseline Plasma TRAP(Before reducing uric acid)
- AFTER Rasburicase Plasma FRAP (Fe⁺² · Lˉ¹)(12 hours after reducing uric acid)