To evaluate antibiogram and occurrence of bacterial biofilm in leprosy plantar ulcers
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 65
- 试验地点
- 1
- 主要终点
- organism for biofilm production
研究概览
简要总结
Sixty-fiveleprosypatients with plantar ulcers attending Dermatology OPD over 24 months period will beenrolled after obtaining informed consent. Swabs will be collected from Plantarulcers. Isolation and identification of bacteria will be performed, based onculture and standard bacterial identification methods. Antimicrobialsusceptibility testing will be conducted using the disk diffusion test.Detection of biofilm production will be carried out using the Micro Titre Platemethod (MTP). The pattern of antibiotic resistance exhibited bybiofilm-producers will be compared with non-biofilm-producers.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 99.00 Year(s)（—）
- 性别
- All
入选标准
- •a) Leprosy patients with plantar ulcers who are not on topical or systemic antibiotics since atleast 2 weeks b) Age above 18 years.
排除标准
- •patients with a history of use of topical or systemic antibiotics within 2 weeks.
- •Patients with ulcer who are admitted in hospital since atleast 5 days which can alter bacterial colonization.
结局指标
主要结局
organism for biofilm production
时间窗: 3 days
measurement of biofilm production by the Microtitre plate method (MTP) will be performed for in clinical isolates of bacteria. An optical
时间窗: 3 days
between 0.120–0.240 a moderate biofilm producer and an OD of less than 0.120,
时间窗: 3 days
Quantitative
时间窗: 3 days
density (OD) greater than 0.240 indicated a strong biofilm producer, an OD
时间窗: 3 days
a non-biofilm producer. S. aureus ATCC 25923 will be used as the control
时间窗: 3 days
次要结局
- The antimicrobial susceptibility testing (AST) determined by the Kirby-Bauer(disc diffusion method after it is incubated for 18–24 h at 37 °C aerobically, the)