Clinicopathological Value of p16 Expression in Colorectal Carcinoma: An Immunohistochemical Study.
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- Evaluation of p16 Expression in Colorectal Carcinoma.
研究概览
简要总结
Evaluation of the immunohistochemical expression of p16 in colorectal carcinoma and correlate its expression to different clinical and pathological parameters as patients' ages, sexes, tumor location, histopathological phenotype, status of nodal involvement, perineural and lymphovascular invasion.
详细描述
Colorectal Carcinoma is very common nowadays, with increasing need for further research.
p16 is tumor suppressor gene with a vital rule ; inactivation of p16 leads to tumor formation and progression.
50 Specimens will be collected from Pathology Department of Sohag University Hospital. Sufficient clinical and histopathological data will be attached for each case.
The previously processed formalin-fixed, paraffin-embedded tissue blocks will be used to get Hematoxylin & Eosin stained sections to confirm the diagnosis and to detect the histopathological type, grade and stage of each tumor.
Another sliced tissue sections will be immunohistochemically stained with p16 monoclonal antibodies to be evaluated with the positivity, intensity and distribution of the detected stain.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Specimens from patients with colorectal cancer.
- •Tissue blocks with sufficient material.
- •Specimens with sufficient clinical data.
排除标准
- •Tissue blocks with insufficient, destroyed or necrotic material.
- •Specimens with insufficient clinical data.
研究组 & 干预措施
Participants
Fifty (50) cases of colorectal cancer will be performed from archieved formalin-fixed, paraffin-embedded tissue blocks referred to Pathology Department, Sohag University Hospitals.
干预措施: Immunohistochemical staining (Biological)
结局指标
主要结局
Evaluation of p16 Expression in Colorectal Carcinoma.
时间窗: 6 months
Immunohistochemical staining of Colorectal tissues by p16 monoclonal antibody
次要结局
- Correlation of p16 expression to different clinical and pathological parameters(6 months)
研究者
Sherry Raouf Azmy
Demonstrator of pathology
Sohag University