CaffeineHSC - Effect of Chronic Caffeine Consumption and Acute Caffeine Administration on the Hematopoietic System
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 100
- 试验地点
- 3
- 主要终点
- Mobilization of hematopoietic stem and progenitor cells
研究概览
简要总结
This study investigates how caffeine intake affects blood stem and progenitor cells in healthy adults.
The trial will compare people who regularly consume caffeine with those who consume very little or none. All participants will receive a single 200 mg caffeine tablet (similar to one cup of coffee) under fasting conditions. Blood samples will be collected before and three hours after caffeine intake.
The study will assess whether caffeine influences the mobilization of blood stem and progenitor cells from the bone marrow into the bloodstream. It will also examine the effects of caffeine on the function, gene activity, and metabolism of blood cells.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Able to give consent.
- •Healthy adults aged ≥ 18 years.
- •Regular caffeine consumers (≥1 coffee cup/day or ≥1 energy drink/day) or non-consumers (≤1 cup/month and ≤1 energy drink/month) for at least 6 months.
- •Willingness to comply with fasting requirements and study procedures.
排除标准
- •Chronic illnesses or medication use affecting metabolism, including metabolic disorders (e.g., diabetes, thyroid dysfunction, metabolic syndrome).
- •Personal history of cancer.
- •Personal history of hematological disorders.
- •History of radiation therapy.
- •Known caffeine hypersensitivity or intolerance.
- •Known immunosuppression.
研究组 & 干预措施
Caffeine intervention
200mg Caffeine
干预措施: Caffeine (200 mg) (Other)
结局指标
主要结局
Mobilization of hematopoietic stem and progenitor cells
时间窗: From baseline to three hours after caffeine intake
The change in the numbers of mobilized HSPCs (defined as CD34+ cells) \[cells/ml\] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers.
Mobilization of hematopoietic stem and progenitor cells
时间窗: From baseline to three hours after caffeine intake
The change in the relative abundance of HSPCs \[% HSPCs of total PBMCs\] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers.
次要结局
- Functional alterations in CD34+ peripheral blood cells(From baseline to three hours after caffeine intake)
- Relative metabolite abundance(From baseline to three hours after caffeine intake)
- Change in the numbers of peripheral blood cells(From baseline to three hours after caffeine intake)
- Functional alterations in CD34+ peripheral blood cells(From baseline to three hours after caffeine intake)
- Transcriptional changes in CD34+ cells(From baseline to three hours after caffeine intake)
- Relative metabolite abundance(From baseline to three hours after caffeine intake)
研究者
Jasmin Rettkowski
Dr. Jasmin Rettkowski, Principal Investigator, Department of Health Sciences and Technology, ETH Zurich
ETH Zurich