Determination of the Levels of Lipocalin-2 and Semaphorin3A in Gingival Crevicular Fluid in Subjects With Different Periodontal Diseases
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- The level of Lipocalin-2
研究概览
简要总结
The aim of this clinical trial is to investigate the concentrations of Lipocalin-2 and Semaphorin 3A levels in gingival crevicular fluid in subjects with different periodontal diseases. At the same time for the periodontitis group; the purpose of this study is to analyze the correlation of these proteins with clinical parameters at the first and third months after the nonsurgical periodontal treatment.
详细描述
In our study, gingival crevicular fluid samples will be taken along with the measurement of all oral clinical parameters from sixty systemically healthy volunteers (20 periodontally healthy, 20 gingivitis, and 20 periodontitis). Gingival crevicular fluid samples will be taken again after the nonsurgical periodontal treatment for the periodontitis group in the first and third months. Lipocalin-2 and Semaphorin 3A levels will be determined by an enzyme-linked immunosorbent assay test (ELISA). Subsequent statistical analyses will be used to interpret protein levels among different groups and peridontitis group after the nonsurgical periodontal treatment.
Possible significant differences may illuminate the future work with Lipocalin-2 and Semaphorin 3A. Also, these glycoproteins may help to develop different diagnostic methods or treatment strategies for future periodontal treatments.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Being systemically healthy
- •Having at least 20 teeth in the dentition, excluding third molars
- •Not using alcohol nor smoking
- •Not using medications
- •Not taking any antibiotics, anti-inflammatory, nor systemic corticosteroid drugs in the last six months
- •Not being in the lactation period nor pregnancy
排除标准
- •Having any oral or systemic diseases
- •Using alcohol or smoking
- •Using medications
- •Taking any antibiotics, anti-inflammatory, or systemic corticosteroid drugs in the last six months
- •Being in the lactation period or pregnancy
- •Being treated with periodontal treatment in the last six months
结局指标
主要结局
The level of Lipocalin-2
时间窗: 18 months
Investigating the concentrations of Lipocalin-2 level in gingival crevicular fluid in subjects with different periodontal diseases.
The level of Semaphorin 3A
时间窗: 18 months
Investigating the concentrations of Semaphorin 3A leves in gingival crevicular fluid in subjects with different periodontal diseases.
次要结局
- Effects of non-surgical periodontal treatment on the levels of Lipocalin-2 and Semaphorin3A(18 months)
研究者
Merve Ceylan
Research Assistant
Izmir Katip Celebi University