Isolation and Characterization of Cancer Stem Cells Using iFP Technique
试验速览
- 阶段
- 不适用
- 入组人数
- 15
- 试验地点
- 1
- 主要终点
- Cancer stem cells percentage
研究概览
简要总结
- to evaluate insoluble fibrinogen particles (iFP), as a tool for harvesting, growing and transferring attachment-dependent cancer stem cells and comparing it to the standard method ( coated plate) .
- to evaluate whether using iFP for growing CSC can yield better results of isolating and enriching CSCs from fresh tumors than other conventional methods
详细描述
There has been a growing interest in recent years on cancer stem cells (CSC) and their implication in cancer biology and therapy.
CSCs refer to a subset of tumor cells that has the ability to self-renew and generate the diverse cells that comprise the tumor.
These cells have been termed cancer Stem cells to reflect their 'stem-like' properties and ability to continually initiate and sustain tumorigenesis.
CSCs share important properties with normal tissue stem cells, including self-renewal (by symmetric and asymmetric division) and differentiation capacity, albeit aberrant. Multilineage differentiation, however, is not a mandatory feature of a CSC. Furthermore, these cells are thought to be resistant to conventional cancer therapy including chemotherapy and radiotherapy which may explain rapid tumor cell repopulation following treatment mainly by this subtype of cells, therefore new therapeutic method to target CSCs are now under thorough investigation.
One of the major difficulties in finding and identifying CSC is that they are hard to isolate, grow, and enrich.
研究设计
- 研究类型
- Observational
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Above 18 years old.
- •Signed ICF.
- •Expected to under go cancer removing surgery in Rambam MC.
- •A sample of the tumor can be brought to the lab within 30 of its removal.
- •Enough histological material to perform a good histological evaluation.
排除标准
- •Patient not interested to participate in clinical trial.
- •PI decision.
- •Chemotherapy or radiotherapy treatment prior to surgery.
结局指标
主要结局
Cancer stem cells percentage
时间窗: 24 months
Evaluation of CSCs and non-CSCs population by using several surface markers. For glioma and pancreas, CSC are defined as CD133 positive. For breast carcinoma CSCs are identified as CD44+/CD24-/Low. Percentage of CSCs will be measured for comparing the yield of the methods using iFP and the standard method.
次要结局
未报告次要终点