Identification of Predictive Markers for Testis Cancer in a Population of Men With High Risk
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 44
- 试验地点
- 2
- 主要终点
- Analysis of 4 recognized tumorogenesis biomarkers in testicular biopsies of a population of hypofertiles men in theory supposed to present an increased incidence of testicular cancer development
研究概览
简要总结
Testis cancer with germ cells is the most frequent cancer of young men and its incidence is in constant increase in many industrialized countries, as in France. An increased risk of developing testis cancer has been described in patients with testicular ectopia history and testicular cancer history (controlateral testicular cancer) and more recently suggested in a population of hypofertile men with altered spermatogenesis. To a better understanding of this risk, an attempt of characterization of this group of patients has been proposed in the present work.
The general objective of this project is to characterize morphological and molecular markers of hypofertility which could serve as predictive markers of testis malignant transformation.
In this project conducted in 3 establishments, the investigators propose:
- To select a population of hypofertile patients exhibiting compatible clinical and morphological characters with a high risk of testis tumoral transformation (secretory azoospermia and/or a history of testicular ectopia. To determine the spermatogenic arrests on histological criteria (score of Jonhsen).
- To study the expression of four proteins or family of proteins suspected of being involved in testis tumorogenesis such as: the Placenta Alkaline Phosphatase (PLAPE), cyclin A1, VASA and connexin (Cx) by immunohistochemistry and by real-time quantitative RT-PCR analysis real-time analyses.
- To establish a possible correlation between the clinical data, spermatogenesis arrest and the expression of these biomarkers.
These approaches would allow to identify, in this population of hypofertile patients, subgroups of men who could develop tumours with germ cells, and subsequently to propose potential biomarkers for testis cancer. A more clinical observation of these subgroups will be also proposed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Patients with infertility azoospermia
- •Age 18 to 35 years
- •Definition of clinical and biological character of azoospermia: testicular volume less than 10 ml and FSH more than 10 IU / l
- •Normal karyotype
- •Surgical exploration included in a standardized medical aid to procreation
- •Testicular biopsy performed by traditional surgery
- •Patient informed about the research protocol and having signed consent to conduct a further testicular biopsy included in this study.
排除标准
- •adults protected by law, minors
- •Individuals who are not affiliated to a social security
- •Subjects hospitalized for any reason other than research.
- •Patients will be used to support reproductive techniques:
- •Positive serology (HIV, hepatitis ...).
- •Patients with karyotype anomalies and unfavourable genetic counselling
结局指标
主要结局
Analysis of 4 recognized tumorogenesis biomarkers in testicular biopsies of a population of hypofertiles men in theory supposed to present an increased incidence of testicular cancer development
时间窗: 4 years
次要结局
- Establishment of one or more correlation (s) between the level of expression of biomarkers of tumorogenesis: Jonhsen score, the rate of circulating spermatogenesis serum markers (AMH, inhibin ...).(4 years)
- Establishment of a correlation between the level of expression of of tumorogenesis testicular biomarkers, the presence of testicular microlithiasis and carcinoma in situ of the testis(4 years)
- Identification of spermatogenic achieving by conventional histology (analysis of Jonhsen's score)(4 years)