Identifying Molecular Determinants of Infertility in Men (MODIFY)
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Semen/Sperm quantity analysis
研究概览
简要总结
The purpose of this study is to understand the underlying mechanisms of infertility caused by unknown factors. The investigator propose to identify small non-coding RNA (sncRNA) biomarkers of infertility and advance towards developing a more accurate and robust approach for infertility diagnosis.
详细描述
The goal of the study is to identify small non-coding RNA signatures of infertility in men through the comparison of sperm sncRNA profiles from around 100 men (21-50 years of age). The participants will be classified into fertile and infertile groups by the conventional method.
Through the project, the investigator intends to understand the underlying mechanisms of idiopathic infertility. By utilizing semen as liquid biopsy, through sperm sncRNA profiling and comparison (infertile vs fertile). The investigator proposes to identify the sncRNA (miRNAs, piRNAs and tRFs) as biomarkers of infertility.
The investigator proposes to generate a validated signature of sncRNAs that will help in selecting high quality sperms for IVF.
研究设计
- 研究类型
- Observational
- 观察模型
- Ecologic Or Community
- 时间视角
- Prospective
入排标准
- 年龄范围
- 21 Years 至 50 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Semen/Sperm quantity analysis
时间窗: 1 week
sperm count and concentration
Fertility classification
时间窗: 1 week
Classification into fertile/infertile groups based on the fertility measures.
Semen/Sperm quality analysis
时间窗: 1 week
semen pH, semen volume, sperm motility, sperm morphology, sperm vitality
Sperm RNA extraction and sequencing
时间窗: 3 months
Sperm RNA extraction and sequencing
次要结局
- Expression profiling of sncRNA(3-6 months)
- Comparison of the expression profiles(3-6 months)
研究者
Candida Vaz
Principal Investigator, Senior research scientist
Institute for Human Development and Potential (IHDP), Singapore