Screening of cancer from peripheral blood sample through different stemcell-based tests
试验速览
- 阶段
- 不适用
- 状态
- Other
- 入组人数
- 500
- 试验地点
- 1
- 主要终点
- Raman Spectroscopy, gene expression patterns of qPCR and rare stem cells
研究概览
简要总结
Aim: Screening of cancer from Peripheral blood sample through different stem cell-based tests
Milestone to be achieved- Collation of data (n=500 subjects including non-cancer individuals and cancer subjects) different phases of study like optimisation and validation.
Objectives:
-
To assess the feasibility of Raman Spectroscopy as a screening tool for cancer detection
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To isolate circulating stem cells from peripheral blood of cancer and non-cancer subjects and determine expression patterns of various markers by quantitative QPCR.
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To isolate and enumerate rare/stem cells from peripheral blood of healthy individuals and cancer patients (Flow Cytometry).
-
To assess the potential of AOB test (Next Generation Sequencing of stem cells/ cancer stem cells/ progenitors enriched from the peripheral blood) for identification of exomic and transcriptomic profile of primary impacted organ.
Exploratory objective:
- To identify the cancer subtype and metastatic site using AOB test.
研究设计
- 研究类型
- Interventional
- 分配方式
- Not Applicable
- 盲法
- Not Applicable
入排标准
- 年龄范围
- 18.00 Year(s) 至 99.00 Year(s)（—）
- 性别
- All
入选标准
- •Healthy individuals:
- •18 years and above healthy individuals will be included in the study.
- •Individuals with no prior history of cancer will be included.
- •Cancer patients:
- •18 years and above cancer patients will be included in the study.
- •Cancer patients will be confirmed on histopathology irrespective of grade and stage.
排除标准
- •Exclusion criteria:
- •Age less than 18 years.
- •Verified biohazard samples will be excluded.
- •Cancer subjects who have received anticancer treatment within 7 days of sample collection
- •Subjects who have undergone surgery within previous 2 weeks of sample collection
- •Pregnant/ lactating females
- •Subjects who have received growth factors in past 2 weeks.
结局指标
主要结局
Raman Spectroscopy, gene expression patterns of qPCR and rare stem cells
时间窗: Baseline
Identification of cancer and non-cancer subjects using biochemical fingerprints of
时间窗: Baseline
enumeration using Flow cytometry.
时间窗: Baseline
• Identification of primary impacted organ using altered gene expression patterns and
时间窗: Baseline
mutations profile wherever applicable.
时间窗: Baseline
次要结局
未报告次要终点