NLRP3 Inflammasome Activation Among Patients During Acute Gout Flares
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- NLRP3 inflammasome activation in white blood cells.
研究概览
简要总结
Gout is a chronic joint disease associated with deposition of monosodium urate crystals, as a consequence of hyperuricemia. Gout is an intermittent flaring condition and acute gout flares are driven by NLRP3 inflammasome and IL1-beta production.
However pathogenesis of acute gout flares remains poorly known in vivo in human.
The aim of this study is to evaluate NLRP3 inflammasome activation in vivo in patients cells during acute gout flares.
详细描述
Blood and synovial fluid will be obtained from remainder of patients' samples during an acute gout flares. White blood cells will be isolated and NLRP3 inflammasome activation will be assessed. Then, NLRP3 inflammasome inhibitors will be tested on cells with NLRP3 inflammasome activity.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Hospitalization for acute gout flare confirmed by joint fluid analysis or Nijmegen score > 8
- •Aged > 18
排除标准
- •Gout treatment already started
- •Inflammatory Arthritis other than Gout Arthritis
- •Pregnant or breast feeding women
- •Patient protected by law
结局指标
主要结局
NLRP3 inflammasome activation in white blood cells.
时间窗: Patient admission at hospital
White blood cells will be isolated from blood sample and NLRP3 inflammasome activation will be assessed by flow cytometry.
次要结局
- NLRP3 inflammasome activation in synovial cells.(Patient admission at hospital)
- Effects of NLRP3 inflammasome inhibitors on proportion of NLRP3 inflammasome activation in white blood cells/synovial cells(up to 7 days after analysis of NLRP3 activity)