Stool Collection From Healthy Donors for the ex Vivo Study of the Mechanism of Action of Active Ingredients on the Intestinal Microbiota: Bacteria / Host Interaction Model
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 4
- 主要终点
- Quantity of Co2 production
研究概览
简要总结
To design and understand the mechanism of action of different combinations of nutraceuticals coupling bacteria, fibers and polyphenols, which can act on the 4 pillars simultaneously via an innovative ex-vivo model approach coupled with functional and quantitative metagenomics.
详细描述
The objective of the study is to collect fresh stools from 4 healthy volunteers, in order to study the mechanism of action of probiotic strains, polyphenols and fibers, alone or in combinations. These fresh stools will be cultured in vitro in fermenters, and the samples taken at different culture times will be analyzed in terms of microbial composition (shotgun metagenomic analysis) and their cellular activity (functional metagenomic analysis).
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Person with a low fiber diet (<17g / day).
排除标准
- •Taking antibiotics and / or transit modulators in the 3 months preceding the sample;
- •Colonoscopy in the last 3 months;
- •Person under legal protection measure (curators, tutors, etc.).
结局指标
主要结局
Quantity of Co2 production
时间窗: Day 1 to Day 15
Change about the quantity of Co2 production after 15 days of incubation
PH evolution
时间窗: Day 1 to Day 15
Change about the PH evolution after 15 days of incubation
Bacterial growth
时间窗: Day 1 to Day 15
Change about the bacterial growth after 15 days of incubation
次要结局
未报告次要终点