Biochemical and Docking Evidence of Neurotransmitter Dysregulation in Specific Learning Disorder
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- Mesurment of serum neurotransmitters levels
研究概览
简要总结
The aim of this clinical trial was to measure how specific neurotransmitter levels in children with Specific Learning Disorder (SLD) change compared to controls. The relationship between these markers and the measured markers will be determined both theoretically and experimentally using various neuropsychological tests. The fundamental questions it aims to answer are
Did neurotransmitter levels increase or decrease in the patient group? Are the neurotransmitter levels measured in the neuropsychological tests related? Can the measured neurotransmitters be used to predict the disease?
Participants:
A single visit to the clinic, a blood sample, and neuropsychological tests will be administered on the same day.
详细描述
The purpose of this investigation was to assess the levels in serum of key neurotransmitters and their precursors (glutamate, L-arginine, and nitric oxide) in children with SLD and also to integrate these biochemical results with molecular docking analyses of their interactions with phospholipase A2 (PLA2) and N-methyl D-aspartate receptors (NMDA), both of which play significant roles in learning function in these patients.
The research group consisted of 20 children aged 7-14 years who had been diagnosed with SLD using DSM-5 criteria, as well as 20 age-matched, gender-matched, and educationally matched healthy controls. The children in the study group underwent the Stroop Test [Total Error Scores, Total Time Scores, Total Correction] and the Wechsler Intelligence Scale for Children (WISC-IV) subtests, and the results were scored. The serum concentrations of glutamate, L-arginine, and nitric oxide (NO) were determined using ELISA. The diagnostic ability of the biomarkers was evaluated using ROC analysis. Additionally, the interactions of glutamate and L-arginine with the PLA2 enzyme and the NMDA receptor were examined using molecular docking analysis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 7 Years 至 14 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Study group: no additional mental disorder except a specific learning disability,
- •The control group had no mental illness.
- •Having no head injury or neurological illness for all groups.
- •There is no drug that might possibly alter cognitive functions for all populations.
排除标准
- •The study group had a mental disease other than a specific learning disability and ADHD.
- •The control group had any mental condition.
- •Having a head injury or neurological illness in all categories.
- •Using drugs that may impair cognitive functioning in all groups those with additional diseases
结局指标
主要结局
Mesurment of serum neurotransmitters levels
时间窗: 6 months
Serum Glutamate Levels (measured in µmol/L by ELISA at baseline) Serum L-Arginine Levels (measured in µmol/L by ELISA at baseline) Serum Nitric Oxide (NO) Levels (measured in µmol/L by ELISA at baseline)
Full Scale IQ (FSIQ)
时间窗: 6 months
Change in Full Scale IQ (FSIQ) score from baseline to 6 months.oints on a scale (range: 40-160; higher scores indicate better performance)
Verbal Comprehension Index (VCI)
时间窗: 6 month
Change in Verbal Comprehension Index (VCI) score from baseline to 6 months.Points on a scale (range: 40-160; higher scores indicate better performance)
Perceptual Reasoning Index (PRI)
时间窗: 6 months
Change in Perceptual Reasoning Index (PRI) score from baseline to 6 months. Change in Perceptual Reasoning Index (PRI) score from baseline to 6 months.
Processing Speed Index (PSI)
时间窗: 6 months
Change in Processing Speed Index (PSI) score from baseline to 6 months. Points on a scale (range: 40-160; higher scores indicate better performance).
Stroop Test Performance
时间窗: 6 months
Change in Stroop Test total errors, total time, and total corrections from baseline to 6 months.Seconds (for time) and number of errors/corrections; lower scores indicate better performance.
Working Memory Index (WMI)
时间窗: 6 months
Change in Working Memory Index (WMI) score from baseline to 6 months. Points on a scale (range: 40-160; higher scores indicate better performance)
次要结局
未报告次要终点
研究者
Dilara Ulger Ozbek
Dr.
Cumhuriyet University