Endothelial Extracellular Vesicles as Biomarkers of Blood Pressure-Induced Endothelial Activation in Adults With Newly Diagnosed Untreated Hypertension
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- Plasma endothelial extracellular vesicle concentration
研究概览
简要总结
Hypertension is associated with endothelial dysfunction, an early event in the development of cardiovascular disease. Endothelial extracellular vesicles (EEVs) have emerged as promising biomarkers of endothelial activation and vascular injury; however, their relationship with blood pressure and microvascular endothelial function in untreated hypertension remains insufficiently understood. This observational case-control study aims to compare circulating EEV concentrations between normotensive adults and adults with newly diagnosed, untreated primary hypertension. In addition, the study will investigate the associations between EEV levels, blood pressure, microvascular endothelial function assessed by laser Doppler flowmetry, and established circulating biomarkers of endothelial activation. The findings may improve understanding of the role of EEVs in early hypertension and support their potential use as non-invasive biomarkers of endothelial dysfunction and cardiovascular risk.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Adults aged 18 years or older.
- •Ability and willingness to provide written informed consent.
- •Normotensive individuals or individuals with newly diagnosed, previously untreated primary hypertension, classified according to the European Society of Hypertension (ESH) guidelines.
- •Ability to comply with all study procedures.
排除标准
- •Secondary hypertension.
- •Previous treatment with antihypertensive medication.
- •History of cardiovascular disease (except newly diagnosed primary hypertension in the hypertensive group).
- •Diabetes mellitus.
- •Chronic kidney disease.
- •Cerebrovascular disease.
- •Peripheral arterial disease.
- •Acute or chronic inflammatory or infectious disease.
- •Active malignancy.
- •Pregnancy or breastfeeding.
- •Current use of oral contraceptives, hormone replacement therapy, or medications known to affect endothelial function.
- •Inability or unwillingness to provide informed consent.
结局指标
主要结局
Plasma endothelial extracellular vesicle concentration
时间窗: Baseline
Concentration of circulating endothelial extracellular vesicles quantified by flow cytometry following size-exclusion chromatography isolation.
次要结局
- PORH response (R-O%)(Baseline)
- Acetylcholine-induced vasodilation (AChID)(Baseline)
- Sodium nitroprusside-induced vasodilation (SNPID)(Baseline)
- VCAM-1 concentration(Baseline)
- ICAM-1 concentration(Baseline)
- Endocan concentration(Baseline)
- Matrix metalloproteinase-9 (MMP-9)(Baseline)
研究者
Ines Drenjancevic
Full Professore Tenure, Head of Department of Physiology and Immunology, Faculty of Medicine Osijek
Josip Juraj Strossmayer University of Osijek