The Effect of Sex Hormone Levels During the Menstrual Cycle on Capsaicin Evoked Cough
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 32
- 试验地点
- 1
- 主要终点
- Emax
研究概览
简要总结
The study aim is to investigate if changes in sex hormones during the menstrual cycle affects cough sensitivity in females and males with mild allergic asthma. Female subjects will be grouped according to natural cycle, or taking oral contraceptives. Male subjects will act as the control group.
详细描述
The study will have a total of 3 visits separated by at least 24 hours. Screening Period (Visits 1) - For All Subjects. Eligible subjects will be identified during the initial screening procedures with measures of spirometry, hyperresponsiveness to methacholine, complete history, physical examination, allergen skin test, and capsaicin cough challenge. For female subjects, this visit will occur during Day 1-5 (follicular phase) of the menstrual cycle. Subjects meeting the eligibility criteria will be randomized to Sequence A or Sequence B.
Sequence A will require Visit 2 to occur during menstrual cycle days 1-5 (follicular phase) and Visit 3 to occur during menstrual cycle days 21 - 25 (luteal phase).
Sequence B will require Visit 2 to occur during menstrual cycle days 21-25 (luteal phase) and Visit 3 to occur during menstrual cycle days 1 - 5 (follicular phase).
During Visit 2 and 3 subjects will undergo measures of spirometry, hyperresponsiveness to methacholine, capsaicin cough challenge, allergen skin test, sputum and blood collection.
Visit Windows Each visit must be separated by a minimum of 24 hours. Study visits can be performed in the morning or afternoon, however for each subject the timing should be consistent with Visit 1 at the same time of day ± 2 hours.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Subjects must be aged between 18 to 35 years
- •Have a diagnosis of atopic asthma (based on at least one positive skin prick test and methacholine PC20 ≤16 mg/ml)
- •FEV1 ≥ 70% of predicted
- •Demonstrate a cough response to capsaicin with a minimum of 3 coughs during the screening capsaicin challenge
排除标准
- •Subjects who are in a pollen season that affects their asthma
- •Subjects who report allergies to capsaicin or bronchoconstrict at the end of the full dose capsaicin cough challenge and require short acting bronchodilator therapy (assess after visit 1)
- •Subjects who do not display evidence of airway hyper-responsiveness (methacholine PC20>16mg/ml) (assess after visit 1)
- •Symptoms of upper respiratory tract infection in the last 1 month which have not resolved.
- •Lower respiratory tract infection or pneumonia in the last 6 weeks.
- •Current smoker or ex-smoker with ≥10 pack year smoking history and abstinence of ≤6 months
- •Asthma exacerbation in the previous month requiring a start of inhaled or oral corticosteroids.
- •Any asthma medication with the exception of infrequent (less than twice weekly) short-acting β2-agonist.
- •Subjects who have changed asthma medication within the past 4 weeks prior to screening
- •A previous asthma exacerbation requiring Intensive Care Unit admission.
- •Significant other primary pulmonary disorders in particularly; pulmonary embolism, pulmonary hypertension, interstitial lung disease, lung cancer, cystic fibrosis, emphysema or bronchiectasis.
- •Pregnancy or breast-feeding
- •Use of ACE inhibitors
- •Any centrally acting medication (opioids, gapapentin, pregabalin, amitriptyline) which in the view of the investigator could alter the sensitivity of the cough reflex
- •History of psychiatric illness, drug or alcohol abuse which may interfere in the participation of the trial.
- •History of current or previous anabolic steroid use in men.
- •For the natural cycle group, the female patients must have regular (24 to 35 day) natural cycles and no oral contraceptive use for ≥6 months
- •For female's taking the oral contraceptive pill they must be taking this ≥6 months
- •Use of B-blockers
- •Patients with clinically significant cardiovascular disease
- •Patients with known allergy to any of the study medications or excipients
- •Patients with a known potentially life threatening allergy (e.g. food allergy, stinging insect allergy), history of anaphylaxis, mastocytosis, angioedema
结局指标
主要结局
Emax
时间窗: One visit during day 1 to day 5 of the menstrual cycle, compared to one visit during day 21 to 25 of the menstrual cycle
The maximum number of capsaicin evoked coughs
次要结局
- ED 50(One visit during day 1 to day 5 of the menstrual cycle, compared to one visit during day 21 to 25 of the menstrual cycle)
- Dose response Curve(One visit during day 1 to day 5 of the menstrual cycle, compared to one visit during day 21 to 25 of the menstrual cycle)
- C2 and C5(One visit during day 1 to day 5 of the menstrual cycle, compared to one visit during day 21 to 25 of the menstrual cycle)