Effect of Branched-chain Amino Acid Supplementation on Muscle Damage in Recreational Athletes
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 78
- 试验地点
- 2
- 主要终点
- Change in maximum voluntary contraction (MVC)
研究概览
简要总结
Seventy-eight recreational athletes who never resistance trained will be randomly assigned into three groups of twenty-six. Branched-chain amino acid will be supplemented for 18days at 0, 200 or 400 mg/body weight(kg)/day in jelly. Participants will be asked to perform elbow flexions on the 12th day of supplementation. Maximum voluntary contractions will be measured before, during and after the supplementation period to compare the effects of different doses of branched-chain amino acid has on muscle damage markers.
详细描述
Previous studies have not been able to show consistent effects due to various reasons. The major limitations are the lack of enough data on the amount and duration of branched-chain amino acid supplementation, and the level of muscle damage for branched-chain amino acid to be effective. Based on a recent meta-analysis, it has been suggested that to be effective, branched-chain amino acid should be supplemented more than 200mg/kg/day before a low-to-moderate intensity exercise bout. Therefore, this study is designed based on the above suggestions and to compare the effect of different doses of branched-chain amino acid on muscle damage markers in healthy recreational active individuals.
Three treatment groups (n=26) will be randomly assigned to be supplemented with either 200mg BCAA/kg/day (BCAA200), 400mg BCAA/kg/day (BCAA400) or placebo (fiber supplement; PL). Supplements will be provided in jelly in a single blind fashion to be ingested 1hour after each of the three main daily meals for 18days. Anthropometric measurements (body weight, lean mass, height), body temperature, ultrasound (US), strength assessment (maximal voluntary contraction, MVC), resting metabolic rate (RMR), rate of perceived exertion (RPE), delayed onset of muscle soreness (DOMS) and blood samples (duplicates; myoglobin; BCAA) will be collected before, during and after the supplementation period at different time points (see appendix for the above tables). Repeated-bout effect (RBE) on both arms will be measured 7 days after the damage inducing session.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Single (Participant)
盲法说明
Participants will not be able to distinguish branched-chain amino acid and fiber supplement by taste, color or scent as the supplements are provided in jelly.
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy males and females at the age of 18-40
- •Endurance trained athletes
- •Weekly exercise duration is less than 150minutes but more than 30 minutes
排除标准
- •Individuals who cannot endure three days for not eating meat
- •Have been performing resistance trainings
- •Have been taking protein supplement
- •Have been consuming a high level of protein in the regular diet
- •Have been taking omega-three and vitamin E supplements
- •Have been taking anabolic Steroids
- •Have been taking regular medications
- •Have cardiovascular Disease
- •Have a history of joint & Muscle Injuries
- •Females with irregular Menstrual Cycle
结局指标
主要结局
Change in maximum voluntary contraction (MVC)
时间窗: baseline (before supplementation) and at 1,3,24,48,72,96,120,144 hours after muscle damage
Participants will be asked to perform three repetitions of concentric elbow flexion at their maximal force (1RM) with both their dominant and non-dominant arm on an isokinetic dynamometer (Biodex).
次要结局
- Change in 3-methyl-histidine(10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in limb girth(10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in body temperature(20 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in muscle soreness(10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in blood myoglobin(10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in ultrasound images of the arm muscles(10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Changes in insulin(10 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in resting metabolic rate(20 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
- Change in range of motion(20 min before muscle damaging exercise and at each of 1,3,24,48,72,96,120,144 hours after muscle damage)
研究者
Peter Lemon
Principal Investigator
Western University, Canada