Effects of Soluble Dietary Fiber on Sport Efficiency and Fatigue Delay in Top Basketball Players
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Rapid microbiota change
研究概览
简要总结
Athlete nutrition is becoming an increasingly important factor on the path to achieving top results. Nutrients such as soluble fiber can alter the intestinal microbiota, resulting in metabolites with a positive effect on metabolism. Thus, the fibers become available, an additional source of energy to the host, have an anti-stress effect and delay the feeling of fatigue. The primary goal of the study is to determine the impact of soluble dietary fiber on increasing athletic efficiency, the secondary goal is to determine the impact of fiber on delaying fatigue. Subjects will be randomly divided into experimental and control group. The sample of variables will consist of genome microbiota genome sequencing data, measures to assess morphological characteristics, lower extremity explosive strength tests, and cardiovascular endurance. The statistical package SPSS will be used for data processing. The expected scientific contribution of this research is to expand the scientific knowledge about the importance of dietary fiber in the diet of athletes with the aim of improving sports performance.
详细描述
Material, participants, methodology and research plan The material will be soluble fiber NUTRIOSE and placebo. Nutriose, derived from corn, is generally well tolerated and no allergic reactions are known. Participants will be made aware of this in informed consent. If, however, there will be any adverse reactions in participant, participant will withdraw from the study immediately.
The participants will be 14 adult, professional basketball players. The sample of subjects was determined by the size of the group in the same training process in order to exclude the influence of different training processes on the research results. Participants who have used antibiotics in the last 3 months, participants with type 1 and 2 diabetes, and also who have used other ergogenic aids in the last 30 days and still using them are excluded.
Participants will be randomly divided into two groups: the experimental group that will consume the tested fibers and the control group that will consume placebo. Design is a double-blind study where an independent statistician will be entrusted with the randomization of subjects into the two groups.
Sample variables:
Methods: dietary methods, anthropometric methods, fatigue assessment questionnaire and fecal samples analysis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Double (Investigator, Outcomes Assessor)
盲法说明
Program is used to make random selection and grouping of participants in two groups: experimental one receiving fiber and control one, receiving placebo. A research assistant who is not involved in study (neither design nor implementation) will pack prebiotic/placebo daily doses (20 grams/day) and provide nutritionist who is one of the investigators with doses for each participant. Only when the study is completed and all results processed will the information on who received fiber/placebo be released.
入排标准
- 年龄范围
- 18 Years 至 22 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Professional basketball players from the same team participating in the same monitored training process
排除标准
- •Diabetes or other chronic disease, taking sports performance enhancing drugs, taking antibiotics 3 months prior or during the course of the study
结局指标
主要结局
Rapid microbiota change
时间窗: 2 weeks
The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. DNA shall be extracted from collected faecal samples, stored at -80°C. DNA extraction shall be performed using the QIAamp® PowerFecal® DNA Kit. DNA concentration shall be quantified using Promega Quantus Flourometer before being sent to amplicon sequencing of variable regions 3 and 4 of the 16S rRNA gene with the primer set 341F (5'-CCTAYGGGRBGCASCAG -3') 806R (5'- GGACTACNNGGGTATCTAAT -3'). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 1 (after 2 weeks) of supplementation shall provide a baseline insight into eventual rapid microbiota change as a response to supplementation/placebo.
Altered microbiota composition
时间窗: 4 weeks
Prolonged supplementation after initial 2 weeks will support the established altered gut microbiota in participants receiving fiber supplement. The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. Gut microbiota DNA shall be extracted from collected faecal samples, stored at -80°C. DNA shall be processed in the same manner as previously described (Outcome 1). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 2 (after 4 weeks) of supplementation shall provide an insight into eventual microbiota change as a response to prolonged supplementation/placebo.
Effect on the perception of fatigue
时间窗: 4 weeks
The major question is whether the moderately increasing fiber intake in athletes has the potential to support the intestinal microbiota and conversely positively impact training-induced perception of fatigue. In order to understand the impact of fiber consumption on the perception of fatigue, after each training session or match, the participants shall be asked to record and send a rating of perceived exertion through a mobile messaging application. The Borg's CR10 RPE scale shall be used for this purpose. This scale has a range from 0 to 10 (RPE - Rating of Perceived Exertion). The RPE scale is a tool used to monitor the perceptual response to training, as a method of determining physical exertion during exercise. The original RPE scale, developed more than 40 years ago, was primarily used to monitor aerobic load, but it still remains widely used in athlete tracking.
次要结局
- Long-term microbiota impact(8 weeks)
研究者
Antonio Starcevic
Associate professor
University of Zagreb