T Cell Repertoire Analysis of Immune Mediated Skin Diseases
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 426
- 试验地点
- 1
- 主要终点
- Peripheral blood will be collected from adults ages 18-85.
研究概览
简要总结
This study is designed to identify the cells of the immune system that cause skin disease such as psoriasis and mycosis fungoides. Blood samples from many patients will be compared in hopes of finding common cells and molecules responsible for skin diseases. Results of this study will increase our knowledge about immune mediated skin disease.
详细描述
The aim of the study is to characterized the T cell repertoire of individuals with immune mediated skin disease (e.g. psoriasis and mycosis fungoides). Peripheral blood with be collected from volunteers with psoriasis, mycosis fungoides and age matched controls. Fifteen tablespoons of blood will be collected prior to the initiation of treatment and again after the patient shows a clinical response to treatment. The time between blood draws will be no less than 3 months. There will be no more than two blood draws per patient. Blood samples will be used to determine the patient's HLA haplotype via PCR and DNA sequencing. After the patient's haplotype has been established the activated T cell repertoire will be analyzed for clonal expansions. Clonal expansions in the T cell repertoire will be determined by immunoscope analysis, which is a PCR based technique.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 55 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Have an immune mediated skin disease, such as psoriasis or mycosis fungoides
- •Are not taking immunosuppressive medications, which may interfere with T cell analysis.
排除标准
- •are taking immunosuppressive medications, which may interfere with T cell analysis
结局指标
主要结局
Peripheral blood will be collected from adults ages 18-85.
时间窗: 2 Years
These samples will then be used for PCR analysis and T cell cloning.
次要结局
- T cell repertoire(2 Years)