Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 22
- 试验地点
- 1
- 主要终点
- Semen parameters after sperm preparation with either method: semen volume
研究概览
简要总结
Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia.
Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Infertile men with astheno-teratozoospermia
- •Women were aged from 18 to 39
- •Women had at least two mature oocytes.
排除标准
- •MI oocytes
研究组 & 干预措施
Density gradient method
Sperm prepared by density gradient method
干预措施: Density gradient (Device)
Microfluidic chip
Sperm prepared by microfluidic chip method
干预措施: Microfluidic sperm sorting chip (Device)
结局指标
主要结局
Semen parameters after sperm preparation with either method: semen volume
时间窗: up to 7 months
semen volume (ml)
Semen parameters after sperm preparation with either method: total motility
时间窗: up to 7 months
total motility (%)
Semen parameters after sperm preparation with either method: progressive motility
时间窗: up to 7 months
progressive motility (%)
Embryo development after sperm preparation with either method
时间窗: up to 7 months
Embryo development and blastocyst formation were evaluated on days 1, 3 and 5 based on the criteria reported by Veeck and Zaninovic, 2003
Semen parameters after sperm preparation with either method: sperm concentration
时间窗: up to 7 months
sperm concentration (106/ml)
次要结局
未报告次要终点