Study of Cancer Stem Cell Vaccine That as a Specific Antigen in Metastatic Adenocarcinoma of the Colorectal
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- The number of participants with adverse events
研究概览
简要总结
Most studies of cancer stem cells (CSC) involve the inoculation of cells from human tumors into immunosuppressed mice, preventing an assessment on the immunologic interactions and effects of CSCs. In this study, the investigators examined the vaccination effects produced by CSC-enriched populations from histologically distinct murine tumors after their inoculation into different syngeneic immunocompetent hosts. Enriched CSCs were immunogenic and more effective as an antigen source than unselected tumor cells in inducing protective antitumor immunity.Immune sera from CSC-vaccinated hosts contained high levels of IgG which bound to CSCs, resulting in CSC lysis in the presence of complement.CTLs generated from peripheral blood mononuclear cells or splenocytes harvested from CSC-vaccinated hosts were capable of killing CSCs in vitro. Mechanistic investigations established that CSC-primed antibodies and T cells were capable of selective targeting CSCs and conferring anti-tumor immunity.
详细描述
To assess the feasibility of generating CSC-loaded DC vaccines for clinical use, the investigators will harvest peripheral blood and tumor specimen from patients with colorectal cancer. The investigators will purify T, B cells and generate DCs from the PBMCs of the colorectal cancer patient.On the other hand, investigators will isolate ALDHhigh and ALDHlow tumor cells from the tumor specimen of the colorectal cancer patient using a similar protocol as investigators reported .
Aim 1: To demonstrate, in vitro, the relative cellular anti-colorectal cancer CSC immunity induced by colorectal cancer CSC-DC primed cytotoxic T cells.
Aim 2: To determine, in vitro, specific binding and lysis of colorectal cancer CSCs by antibodies produced by purified B cells from PBMCs stimulated with colorectal cancer CSC-DC.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Investigator)
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age:18 and over
- •Performance status:Karnofsky 70-100%
- •Life expectancy:Greater than 6 months
- •Hematopoietic:Absolute neutrophil count at least 1000/mm 3,Hemoglobin at least 9 g/dL,Platelet count at least 100,000/mm^3
- •Hepatic:Bilirubin less than 2.0 mg/dL No chronic or acute hepatic disease Renal:Creatinine less than 2.5 mg/dL
- •Cardiovascular:
- •No chronic or acute cardiac disease (New York Heart Association class III or IV)
- •No chronic or acute pulmonary illness such as asthma or chronic obstructive pulmonary disease
排除标准
- •Patients receiving anticoagulation therapy.
- •Patients receiving any other investigational agents.
- •Patients who test positive for Hepatitis B virus, Hepatitis C virus or HIV. 4、level 3 hypertension; 5、severe coronary disease; 6、 myelosuppression; 7、 respiratory disease; 8、 brain metastasis; 9、 chronic infections
研究组 & 干预措施
non-cancer stem cell vaccine
This is no cancer stem cells vaccine in this group
干预措施: cancer stem cell vaccine (Biological)
giving low dose vaccine
The using dosage,frequency and duration of cancer stem cell vaccine are still undetermined.
干预措施: cancer stem cell vaccine (Biological)
giving middle dose vaccine
The using dosage,frequency and duration of cancer stem cell vaccine are still undetermined.
干预措施: cancer stem cell vaccine (Biological)
giving high dose vaccine
The using dosage,frequency and duration of cancer stem cell vaccine are still undetermined.
干预措施: cancer stem cell vaccine (Biological)
结局指标
主要结局
The number of participants with adverse events
时间窗: up to 3 months
次要结局
- The secondary objectives are to evaluate vaccine immune responses to the immunizations by the data of body measurements(1 month)