The Effect of Culture Media Microdroplet Geometry and Different Oil Overlay on Pre Implantation Embryonic Development in Dry Incubators: Prospective Randomized Study.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 100
- 试验地点
- 2
- 主要终点
- Blastulation rate
研究概览
简要总结
A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.
详细描述
Purpose:
to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators
Method:
We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions.
statistical analysis done using SPSS version 23
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 37 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Female age is ≤ 37 years old with ≥ 8 MII oocytes
- •Males with normal semen parameters (WHO 2010).
- •We conducted three experiments all with Siblings oocytes splits and all embryos
排除标准
- •Oocyte or sperm donation.
- •Sever male factor.
- •Any contradictions to undergoing in vitro fertilization or goandotropin stimulation.
结局指标
主要结局
Blastulation rate
时间窗: 5-6 days after ICSI
Percentage of total blastocysts / Total cleaved embryos
High quality blast rate
时间窗: 5-6 days after ICSI
Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A and B ( A is the highest quality). Total high grade embryos/ total blast embryos.
次要结局
- Fertilization rate(16-19 hours of ICSI)
- Cleavage rate(72 hours of ICSI)
- High quality D3 embryos(72 hours of ICSI)
- Good quality trophectoderm/inner cell mass(5-6 days after ICSI)