The Effect of Dietary Macronutrient Manipulation and Glycogen Depleting Exercise on Substrate Oxidation at Rest and During Exercise
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 13
- 试验地点
- 1
- 主要终点
- Muscle glycogen concentration after dietary macronutrient manipulation
研究概览
简要总结
This study will investigate the effect of dietary macronutrient manipulation on glycogen (stored carbohydrate) levels in the muscle and substrate oxidation during exercise. The investigators will investigate the hypothesis that manipulating the macronutrient content of the diet will alter the levels of glycogen in the muscle and, in turn, substrate oxidation during exercise.
详细描述
One way to help reduce body fat is to expend more energy from the oxidation of fat as a fuel. Exercise is one way to increase fat oxidation. The extent to which exercise facilitates the use of fat as a fuel may be influenced by an individual's dietary intake.
This study aims to establish the effect of dietary macronutrient manipulation on muscle glycogen levels and substrate oxidation during exercise. Participants will undertake three separate trial periods. During each trial period participants will consume a diet containing different macronutrient compositions. For each of the trials the investigators will assess how the macronutrient composition of the diet affects muscle glycogen level and substrate oxidation during exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Good general health
- •Non-smoking - currently and for the past 5 years
- •Fitness - V̇O2max 40-60 ml/kg/min
- •Body mass index (20-25 kg/m2)
排除标准
- •Known or suspected intolerance to the dietary interventions or their stated ingredients.
- •Participation in another clinical study or receipt of an investigational drug within 30 days of the screening visit.
- •Previous participation in this study.
- •Recent history (within the last year) of alcohol or other substance abuse.
- •An employee of the sponsor or the study site or members of their immediate family.
- •Abnormal / extreme habitual dietary practice / intake
- •Recent (≤3 months) dietary practice that may be deemed as low and/or high in macronutrients will be excluded.
- •The taking of any prescribed medication
研究组 & 干预措施
Experimental arm 1
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Diet with a different macronutrient content A (Other)
Experimental arm 1
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Interval treadmill running exercise (Other)
Experimental arm 1
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Steady state treadmill running exercise (Other)
Experimental arm 2
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Diet with a different macronutrient content B (Other)
Experimental arm 2
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Interval treadmill running exercise (Other)
Experimental arm 2
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Steady state treadmill running exercise (Other)
Experimental arm 3
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Diet with a different macronutrient content C (Other)
Experimental arm 3
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Interval treadmill running exercise (Other)
Experimental arm 3
One of three 7-day study arms consisting of 3 days of standardised diet followed by 3 days of dietary macronutrient manipulation and 1 day of experimental testing.
干预措施: Steady state treadmill running exercise (Other)
结局指标
主要结局
Muscle glycogen concentration after dietary macronutrient manipulation
时间窗: Day 7 of all experimental arms
Muscle glycogen concentration will be measured after the dietary macronutrient manipulation intervention.
次要结局
- Blood insulin concentration(At 0, 20, 40 and 60min into exercise on day 7 of all experimental arms)
- Rate of fat oxidation during exercise (g/min)(During 60 min of exercise on day 7 of all experimental arms)
- Rate of carbohydrate oxidation at rest (g/min)(For 20 min on Day 7 of all experimental arms)
- Rate of carbohydrate oxidation during exercise (g/min)(During 60 min of exercise on day 7 of all experimental arms)
- Rate of fat oxidation at rest (g/min)(For 20 min on Day 7 of all experimental arms)
- Blood glucose concentration(At 0, 20, 40 and 60min into exercise on day 7 of all experimental arms.)
- Blood fatty acid concentration(At 0, 20, 40 and 60min into exercise on day 7 of all experimental arms)
- Change in intramuscular triglyceride (IMTG) content after exercise(IMTG content measured just before and immediately after 60min of exercise on Day 7 of all experimental arms)