Effect of Vitamin D Deficiency on the Structure of Titanium Platelet-Rich Fibrin(T-Prf)
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- microscopic analysis
研究概览
简要总结
ABSTRACT The aim of the study was to compare the macroscopic properties, chemical degradation levels, and fibrin network structures of T-PRF membranes obtained from systemically healthy individuals with optimal vitamin D levels, with vitamin D-deficient individuals who reached optimal levels after three months of vitamin D supplementation.
A total of 48 participants, 24 with vitamin D deficiency and 24 with optimal vitamin D levels, were included in the study. The macroscopic properties (weight, width, length, density), chemical degradation levels and fibrin network structures of the obtained T-PRF membranes were evaluated using scanning electron microscopy (SEM) and light microscopy.
详细描述
Blood samples taken from individuals with the help of a 20 ml syringe were immediately transferred to 2 10 ml titanium tubes and placed opposite each other to ensure balance in the tabletop centrifuge device in order to be properly separated in the centrifuge process. To obtain T-PRF, it was centrifuged in the IntraSpin™ device at 2700 rpm centrifugal force for 12 minutes.
After centrifugation, the fibrin in the middle of the 3 layers formed in the titanium tube was held and pulled with the help of a press. Then, the red layer in the lower layer, where erythrocytes were dense, was separated from the fibrin layer with the help of scissors and the membrane was obtained by pressing for 2 minutes using the own weight of the PRF box.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Double (Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Individuals aged 20-55
- •Being systemically healthy
- •Not using any medication regularly
- •Not taking additional vitamin D from outside
- •Patients who met the conditions of not having used antibiotics for the last three months were included in the study.
排除标准
- •People who use cigarettes (tobacco) and alcohol
- •Those with platelet function and clotting disorders
- •Those who are pregnant or breastfeeding
- •Those who use medications that affect the natural clotting process
- •Those with a history of infection in the last three months
- •Those receiving immunosuppressive therapy
- •Those who do not consent to volunteer
研究组 & 干预措施
with vitamin D deficiency
To obtain T-PRF membranes, blood was taken from people diagnosed with vitamin D deficiency. After 3 months, blood was taken from individuals taking vitamin D again to obtain T-PRF membranes.
干预措施: Vitamin D (Cholecalciferol ) (Dietary Supplement)
Vitamin D level is optimal
Blood was taken from individuals with optimum vitamin D levels to obtain T-PRF membranes
结局指标
主要结局
microscopic analysis
时间窗: From enrollment to the end of treatment at 3 months
T-PRF membranes were examined histologically using the cell block cytology method. Membranes were transferred to cassettes and fixed in formalin solution for 24 hours. At the end of 24 hours, the cassettes were subjected to dehydration with various concentrations of formalin, alcohol and xylene. The tissue was embedded in Leucher blocks using paraffin so that it could be easily cut and examined. 3 µm thick sections were taken from the embedded blocks using a microtome. Paraffin in the blocks was removed and the tissue was stained with hematoxylin-eosin L-PRF clot was analyzed microscopically on hematoxylin-eosin stained slides using the blood element adhesion index score 0: absence of fibrin network score 1: sparsely dispersed fibrin network score 2: thin fibrin network poorly interlocking score 3: dense fibrin network abundantly interconnected
次要结局
- Scanning Electron Microscope (SEM) Examination(From enrollment to the end of treatment at 3 months)
- T-PRF Degradation of Membranes(From enrollment to the end of treatment at 3 months)
研究者
Esra ATEŞ
Assistan professor
Abant Izzet Baysal University