Effect of Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice on Telomere Length in Peripheral Blood Leukocytes A Follow-up Pilot Study
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 发起方
- 入组人数
- 12
- 试验地点
- 1
- 主要终点
- Telomere Length in Peripheral blood leukocytes
研究概览
简要总结
Effect of Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice on Telomere Length in Peripheral Blood Leukocytes A Follow-up Pilot Study
The goal of this Pilot clinical trial is to learn if a Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice works to increase Telomere Length in Peripheral Blood Leukocytes in adults. The main questions it aims to answer are:
- Does A Subtle energy transmission and Tao Calligraphy Mindfulness increase Telomere Length in Peripheral Blood Leukocytes in adults?
- Will this increase of Telomere Length in Peripheral Blood Leukocytes in adults be statistically significant? Researchers will compare the length of Telomere in peripheral blood Leukocytes at beginning of the mindfulness practices to the length of Telomere in peripheral blood Leukocytes at 3 months and at 9 months of regular daily practices.
Participants will:
- Receive a transmission of Subtle energy at beginning of practices
- Visit the Laboratory for a blood sample taken at beginning, at 3 months and at 9 months of practices.
- Fill the Study Questionnaires at beginning, at 3 months and at 9 months of practices.
- Practice the Mindfulness with Tao Calligraphy daily 1 hour in the morning and 1 hour in the evening.
详细描述
OBJECTIVES The proposed research is a pilot project based on experiential data obtained from several previous pilot and formal studies by the investigators' research teams and by researchers from other institutions. It is intended to measure the effects of the transmission of subtle energy (blessing), combined with a specific form of meditation, on the length of human telomeres in peripheral blood leucocytes and on overall well-being. The investigators hope that the study will provide further data for individuals seeking an additional modality to prolong life expectancy that could be integrated into conventional medical protocols.
HYPOTHESIS The research null hypothesis is that individuals who receive a subtle energy transmission for improving telomere length in peripheral blood leucocytes and who will also then regularly practice mindfulness with Tao Calligraphy for nine months will (a) have no significant change in telomere length in follow-up analyses as measured by telomere length in peripheral blood leukocytes and will (b) show no improvement of well-being as assessed via the standardized scientific questionnaire Rand SF 36(s) at three and nine months.
Statistical test Anova (using Minitab version 14 and PSPP/SPSS) will be used to evaluate the null hypothesis. The p-value will represent how unlikely the observed data would be if the null hypothesis were actually true and the investigators will use them to reach conclusions. The investigators set the confidence level at 95% and if p <= 0.05, then H0 is rejected.
BACKGROUND Telomeres as Markers of Longevity Background: Telomeres are distinctive protein-DNA structures (repetitive (TTAGGG) sequences) at the end of the chromosomes that are essential for maintaining the integrity and stability of the genome. Telomere length shortens with age (due to multiple cell divisions). The rate of telomere shortening has been found to increase or decrease in association with specific lifestyle factors.
To counteract the loss of telomere repeats with each cell division, germline cells, highly proliferating cells from the skin, the gut, and hematopoietic tissues, as well as tumor cells, express DNAmAge telomerase, an enzyme complex with reverse transcriptase activity, which adds the necessary telomeric DNA (T2AG3 repeats) to the 3-end of the telomeres, protecting their degeneration. Despite expression of telomerase, the telomere length in highly proliferating cells from long-lived species decreases during development and with replication in vitro and with age in vivo. Most likely, telomerase levels in such cells are tightly regulated and are insufficient to make up for the net loss of telomere repeats with cell division.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Supportive Care
- 盲法
- None
入排标准
- 年龄范围
- 60 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adult subjects / patients (age 60 years and over) we expect at 60 years people have already measurable shortening of telomeres
- •Healthy or Ill, with the exception of genetic illnesses and cancer (for which treatments could negatively impact telomeres)
- •Willingness and ability to comply with data collection requirements.
- •Complete submission of required documentation prior to enrollment into the study, including informed consent and consent to release of information.
- •Willingness to allow their data to be used for research purposes and published as deemed fit (while conforming to all applicable privacy laws) by Sha Research Foundation.
- •Willingness to practice the daily calligraphy meditations and follow the protocol.
排除标准
- •Not meeting any of the inclusion criteria
- •Bipolar disorders, other serious mental disorders (e.g. schizophrenia, psychosis), genetic illnesses (primarily affected chromosomes), and cancer (treatment could negatively impact telomere during research period)
- •inability to sign consent and follow instructions
- •Unwillingness to participate in data gathering
- •Unable to follow the practice regimen, including the daily calligraphy meditations
- •Pregnant or nursing. Participants who become pregnant during the study will be required to end their participation. (to avoid any, at current time unknown, potential negative effect of the study on the fetus).
- •There are no exclusion criteria placed upon potential subjects related to national origin, culture, ethnicity, race, sex, physical disability, sexual orientation, religion, or spiritual practices.
研究组 & 干预措施
SIMPLE FOLLOW UP GROUP
Every subject who is accepted into the study, will receive prior to the inception of practice transmission of pure energy (blessing) and will be instructed on how to practice meditations with Tao Calligraphy.
Blood samples will be drawn at DAP accredited institution in British Columbia (Life Labs or Vancouver Coastal Health) and will be promptly sent by currier to Repeat Diagnostics, All participants will have blood samples drawn and Questionnaire filled at the entry time point (Time 0), at the end of 3 months (Time 1), and at the end of 9 months (Time 2).
干预措施: Mindfulness practice with Calligraphy (Other)
结局指标
主要结局
Telomere Length in Peripheral blood leukocytes
时间窗: 9 months
The Change of Telomere Length in Peripheral blood leukocytes after 9 months of Mindfulness Practice with Tao Calligraphy
次要结局
- Quality of Life by John Ware's SF-36 Quality of Life questionnaire (that produces eight scores with 0-to-100 scale, 100 being the best possible outcome).(9 months)
研究者
Peter Hudoba De Badyn MD, FRCS
Director of the Research at Sha Research Foundation
Sha Research Foundation