Development of a Novel Functional Food Enriched With Beta-glucans Isolated From Edible Mushrooms of Greek Habitats
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Change from baseline of barrier function (paracellular permeability) after 90 minutes of ex vivo stimulation of the colonic biopsies.
研究概览
简要总结
The aim of the present study was to investigate the ability of Pleurotus eryngii mushrooms fermentation products (FS) to counteract induced intestinal hyperpermeability in human colonic tissues in an ex vivo system.
详细描述
Collection of colon biopsies through sigmoidoscopy procedure will take place and the collected biopsies will be mounted in Ussing Chambers. Already collected fermentation supernatants will be added to the mucosal side of the biopsy together with a stressor and two permeability markers, in order to investigate the effects of the fibre fractions on both paracellular and transcellular permeability.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Signed consent prior to any study related procedures
- •Age 18-65 years
- •Willing to abstain from regular consumption of prebiotics/probiotics products or medication known to alter gastrointestinal functions at least 4 weeks prior to the study visits
排除标准
- •Previous complicated gastrointestinal surgery
- •Presence of gastrointestinal disorder or any disorder which the principal investigator considers to affect the results of the study
- •Current diagnosis of psychiatric disease
- •Current and past diagnosis inflammatory gastrointestinal disease (e.g. Irritable Bowel Disease)
- •Systemic use of antibiotics or steroids medications in the last 3 months
- •Frequent use of NSAID (Non Steroidal Anti Inflammatory Drugs) the last 2 months prior to study visits
- •Regular consumption of prebiotic/probiotic products for the past 4 weeks
- •Abuse of alcohol or drugs
- •Frequent use of laxatives, anti-diarrheal, anti-cholinergic within last 12 weeks prior to study visits
- •Pregnancy and breast-feeding
结局指标
主要结局
Change from baseline of barrier function (paracellular permeability) after 90 minutes of ex vivo stimulation of the colonic biopsies.
时间窗: Barrier function will be measured at baseline and after 90 minutes of ex vivo stimulation of the colonic biopsies.
Barrier function (paracellular permeability) will be evaluated with the use of marker related this permeability, through immunofluoresence.
Change from baseline of barrier function (transcellular permeability) after 90 minutes of ex vivo stimulation of the colonic biopsies.
时间窗: Barrier function will be measured at baseline and after 90 minutes of ex vivo stimulation of the colonic biopsies.
Barrier function (transcellular permeability) will be evaluated with the use of marker related to this permeability, through ELISA tecnhique.
次要结局
未报告次要终点