TUSEB-Nasopharynx Microbiota Component and in Vitro Cytokines Production in Coronavirus Disease (COVID-19)
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Determination of peripheral blood RNA microarray assay
研究概览
简要总结
The investigators will study nasopharunx microbiota composition, RNA sequences and in vitro cytokines production by peripheral blood cells of COVID-19 patients (both mild-moderate and severe cases) (comparing with convalescent patients and healthy controls).
详细描述
The investigators will compare study parameters (basal) between mild-moderate and severe COVID-19 patients and with other study groups (i.e. convalescent and healthy controls). Nasopharunx microbiota dysbiosis, in vitro cytokines production profile, and peripheral blood flocytometric results of the four study groups will be compared.
In vitro cytokines production will be studied and compared in Nill (No antigens) and Mitojen (Cocktail of antigens) pre-prepared test tubes.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 90 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Ability to give a written consent
排除标准
- •In ability to give a written consent
- •Using of medications that affect the results of study parameters
- •Presence of malignancy or tuberculosis
结局指标
主要结局
Determination of peripheral blood RNA microarray assay
时间窗: First day
Four study groups, microarray Analysis .
Determination of nasopharunx Microbiota analysis
时间窗: 1st day
Four study groups, metagenomic analysis, to determine abundance and decrease of some species.
Determination of in vitro cytokines production
时间窗: First day
IL-2, IL-7, IL-10, granulocyte-colony stimulating factor, interferon-p inducible protein 10, monocyte chemo-attracting protein 1, macrophage inflammatory protein 1-α and tumor necrosis factor-α in Nill (no angijen) and Mitojen (Cocktails of antigens)tubes (between 4 study groups)
Determination of peripheral blood cytometry
时间窗: First day
Four study groups, peripheral blood cytometry; CD3, CD4, CD8, and CD45 rates.
Relation between study parameters
时间窗: First day parameters
determining the realtion between microbiotme dysbiosis, in vitro cytokines production and peripheral leymphocytes (CD3,CD4, CD8, and CD45) rates and the severity of COVID-19( if any)
次要结局
未报告次要终点
研究者
Mehmet Hursitoglu
Associate Professor
Başakşehir Çam & Sakura City Hospital