PTP1B Implication in the Vascular Dysfunction Associated With Obstructive Sleep Apnea
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 58
- 试验地点
- 2
- 主要终点
- myeloid PTP1B expression
研究概览
简要总结
Obstructive sleep apnea (OSA) syndrome is associated with increased vascular dysfunction and atherosclerosis. Especially, it has been shown that OSA associated intermittent hypoxia represents a pro inflammatory stimulus resulting in macrophage polarization.
Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin signaling pathways involved in atherosclerosis. It has been shown that myeloid PTP1B deficiency protects against atherosclerosis.
As hypoxia has also been shown to increase PTP1B expression and activity, this study will evaluate the myeloid PTP1B expression and activity in patients with OSA as compared to controls and will investigate myeloid PTP1B involvement in the vascular pro inflammatory precess described in OSA.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Overnight polysomnographic recording for suspected OSA
- •Apnea hypopnea index ≥ 15 per hour for OSA patients
- •Apnea hypopnea index < 15 per hour for non OSA patients
排除标准
- •past history of cardiovascular diseases
- •dyslipidemia
- •hypertension
- •obesity (body mass index > 35 kg/m2
- •past history or ongoing inflammatory diseases
- •cognition impairment
结局指标
主要结局
myeloid PTP1B expression
时间窗: baseline
Peripheral blood will be collected from OSA and non OSA patients. Macrophage will be isolated by specific centrifugation. PTP1B expression will be assessed by Western Blot.
myeloid PTP1B activity
时间窗: baseline
Peripheral blood will be collected from OSA and non OSA patients. Macrophage will be isolated by specific centrifugation. PTP1B activity will be assessed by specific colorimetric assay.
次要结局
- in vitro PTP1B implication on macrophage inflammatory response(baseline)
- PTP1B exosomal expression.(baseline)