Association Between the Expression Levels of MicroRNA-101-3p and Autotaxin in Serum of Systemic Lupus Erythematosus Patients
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 40
- 主要终点
- Serum levels of miR-101-3p and Autotaxin in SLE patients versus healthy controls
研究概览
简要总结
Systemic lupus erythematosus (SLE) is a complex autoimmune disease affecting multiple organs and characterized by heterogeneous clinical manifestations. This case-control study aims to assess the association between serum expression levels of MicroRNA-101-3p and Autotaxin (ATX) in SLE patients compared with healthy controls. The hypothesis is that dysregulation of miR-101-3p and ATX contributes to SLE pathogenesis and may serve as potential non-invasive biomarkers for disease activity and prognosis.
详细描述
This descriptive case-control study will enroll 40 SLE patients fulfilling the 2012 Systemic Lupus International Collaborating Clinics (SLICC) classification criteria and 40 age- and sex-matched healthy individuals. Demographic, clinical, and laboratory data will be collected, including disease duration, activity indices (SLEDAI-2K), and damage index (SDI). Blood samples will be taken for biochemical and molecular analyses. Serum miR-101-3p expression will be quantified by qRT-PCR, while Autotaxin and IL-6 levels will be measured by ELISA. Statistical correlations between these biomarkers and clinical variables will be evaluated. The study aims to identify diagnostic and prognostic biomarkers that may guide future therapeutic targets in SLE.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Study Population: Adult patients diagnosed with systemic lupus erythematosus and healthy matched controls.
- •Inclusion Criteria:
- •- Age ≥ 18 years
- •Diagnosis of SLE according to 2012 SLICC criteria
- •Willingness to participate and provide informed consent
排除标准
- •Age < 18 years
- •Pregnancy or lactation
- •Active infections or malignancy
- •Co-existing autoimmune diseases
- •Refusal to participate
结局指标
主要结局
Serum levels of miR-101-3p and Autotaxin in SLE patients versus healthy controls
时间窗: 6 months (expected)
To determine the difference in serum expression levels of miR-101-3p and Autotaxin between patients with systemic lupus erythematosus (SLE) and age- and sex-matched healthy controls using qRT-PCR and ELISA.
次要结局
- Correlation between miR-101-3p and Autotaxin levels(6 monthes expected)
研究者
Eman Mohamed Shawky
Lecturer of Rheumatology and Rehabilitation, Faculty of Medicine, Assiut University
Assiut University