Study to Investigate Signaling Pathways in Placentas of Overweight or Obese Patients and Patients With Gestational Diabetes Mellitus
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 247
- 试验地点
- 1
- 主要终点
- Activity of Wnt signaling pathway in placental tissue
研究概览
简要总结
Aim of the study was to determine if signaling pathways in placentas of mothers affected by overweight/obesity or by gestational diabetes are altered compared to placentas of a control group (normal weight, no gestational diabetes). Moreover, maternal blood and umbilical cord blood were analysed.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •singleton pregnancy
- •BMI before pregnancy >= 18
- •with & without gestational diabetes
- •c-section at gestational week 35+0 until 41+6
排除标准
- •multiple pregnancy
- •maternal serious internal disease
- •infections before pregnancy (e.g. HIV, hepatitis B)
- •pregnancy-associates diseases (e.g. gestoses, androgen insensitivity syndrome)
结局指标
主要结局
Activity of Wnt signaling pathway in placental tissue
时间窗: through study completion, about 4 years in total
Analysis of components of the Wnt signaling pathway; like LRP6 receptor, beta-Catenin, GSK3beta, Wnt ligands (=proteins of interest) and an appropriate protein for normalization. Total protein lysates of placental tissue will be used. Analysis will be performed by western blotting and subsequent densitometric analysis. Outcome measure will be \[relative protein level of protein or interest/relative protein level of normalization protein\].
次要结局
未报告次要终点
研究者
Sarah Appel
Principal Investigator
Universitätsklinikum Köln