Investigation of Relation Between Demodex Density With Immune Response and Oxidative Stress in Rosacea Patients
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- Severity of rosacea
研究概览
简要总结
Aim of study is to determine reactive oxygen products and antioxidant capacity with rosacea patients; to measure metalloproteinase level and inflammatory markers of immune response and investigate their corelation with demodex infestation and also investigate the role of photoaging and sebum secretion in rosacea.
详细描述
Rosacea is a common and chronic inflammatory disease characterized by facial erythema, papule, pustule and telangiectasia. Ultraviolet, Demodex mite, oxidative stress and antioxidant system disorders are emphasized in increased inflammation seen in rosacea.
The aim of our study was to evaluate the status of oxidative stress, matrix metalloproteinase (MMP) and inflammatory markers in systemic circulation, their relationship with demodex density and investigate the role of photoaging and sebum secretion in rosacea.
Forty patients with rosacea and age, gender and skin phenotype matched 40 healthy volunteers were enrolled in the study. Reflectance confocal microscopy (RCM) was used to calculate facial demodex density. The number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM.
Photoaging severity and facial sebum levels were also assessed. Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Between the ages of 18-70
- •No systemic illness
- •No other inflammatory dermatoses except for rosacea
- •No smoking
- •Not using any topical, systemic treatment and sunblock at least a month for rosacea or other condition
排除标准
- •Systemic illness
- •Other inflammatory dermatoses except for rosacea
- •Using any topical, systemic treatment and sunblock for rosacea or other condition
- •Type 4 rosacea patient (only ocular involvement)
结局指标
主要结局
Severity of rosacea
时间窗: Rosacea patients who refer to criteria will be evaluated throughout 4 months.
次要结局
- Paraoxonase-1 (PON-1)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- The number of demodex of right cheek and forehead(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- The number of follicle of right cheek and forehead(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- The number of mite per infested follicle of right cheek and forehead(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Total oxidant capacity (TOC)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Aryl esterase (ARES)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Tumor necrosis factor-alpha (TNF-α)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- The number of mite per follicle of right cheek and forehead(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- The number of infested follicle of right cheek and forehead(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Total antioxidant capacity (TAC)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Oxidative stress index (OSI)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Myeloperoxidase (MPO)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Interleukin-1beta (IL-1β)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Matrix metalloproteinase-1 (MMP-1)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
- Matrix metalloproteinase-1 (MMP-9)(Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.)
研究者
Tuğba Falay
Resident Doctor
Istanbul Training and Research Hospital