Case Control Study of the Risk Factors for Pressure Ulcers in Tunisian Patients

Anthropometric characteristics

时间窗: one hour

Body Mass Index (BMI) is a simple index of weight-for-height. It is defined as the weight in kilograms divided by the square of the height in metres (kg/m2).

Antioxidant parameters

时间窗: one day

- Serum catalase activity in KU/l was determined according to the spectrophotometric method of Goth .

Dyslipidemia

时间窗: one hour

* Lipid markers: total serum cholesterol (CH), serum triglyceride, serum HDL- CH, in mmol/l, levels were measured by standard enzymatic methods using reagents in a fully automated analyzer (Randox Antrim, UK; CX9-BECKMANN). * Low density lipoprotein cholesterol (LDL-C) in mmol / l was determined by Friedewald formula. * non esterified fatty acids in serum was determined by colorimetric method at 550 nm (mmol/l)

Renal failure

时间窗: one hour

- renal profile: urea (mmol/l), creatinine and uric acid (μmol/l) levels were measured by standard enzymatic methods using reagents in a fully automated analyzer ( Cx9 Pro-Bechman Coulter-Fuller-Ton).

Endogenous inflammatory marker

时间窗: one hour

- α1-acid glycoprotein, in g/l, measured using the dry chemistry method (BN prospec, siemens)

Marker of lipid peroxidation

时间窗: one day

* Serum total homocysteine concentrations in μmol/l were measured by using an AxSYM (ABBOTT) homocysteine assay. * thiobarbituric acid reactive substances (TBARS) in serum was determined by the fluorimetric method of Yagi in μmol/l.

Total antioxidant status

时间窗: one hour

Serum total antioxidant status in mmol/l was measured with RANDOX kit (Cat. No. NZ 2332; Randox Labs Ltd., Crumlin, UK) by colorimetric method at 600 nm .

Determination of trace elements

时间窗: one hour

Serum copper in μmol/l was indicated spectrophotometrically with RANDOX kit (Cat. No. CU 2340; Randox Labs Ltd., Crumlin, UK) at 580 nm according. Serum zinc was measured in μmol/l with RANDOX kit (Cat. No. ZN 2341; Randox Labs Ltd, Crumlin, UK) at 560 nm.

Nutritional risk

时间窗: 3 hours

- Prognostic Inflammatory and Nutritional Index (PINI) is a simple clinical \[PINI = AAG x CRP / albumine x prealbumin\] and classificated as follows: normal (1\<PINI score \<10), mild malnutrition (11\<PINI score\<20), severe malnutrition (21\<PINI score\<30) and risk for death when PINI score \>30. These scores gained in popularity as it uses an objective rather than subjective measurements to determine nutritional risk in hospitalized patient populations.

DNA extraction

时间窗: 2 days

Genomic DNA was extracted from whole blood using the salting out method for the part of molecular biology.

Nutritional status

时间窗: 3 hours

- Nutritional Risk Index (NRI) was originally derived from the serum albumin concentration and the ratio of present to usual weight \[NRI = (1.489 x albumin, g/L) + (41.7 x present weight/ideal body weight)\] and categorized as follows: severe risk (NRI \< 83.5), moderate risk (83.5 \< NRI \< 97.5) and no risk (NRI \> 97.5).

A microbiological diagnosis

时间窗: 3 days

The bacterial colonization of a wound is a recognized detrimental factor in the multifactorial process of wound healing. wound per patient suffering from pressure ulcer was cultured by swab to determine the bacterial species of the infection and helps guide antibiotic therapy. The representative sample is collected before topical or systemic antibiotics are initiated and pain assessment should be conducted prior to wound procedures such as dressing changes and debridement. Bacterial swabs provide information on the predominant flora.

Proteomics

时间窗: 2 days

- Serum gelatinase activities of MMP-9 by zymography (%)

Genotype for the MMP9-1562 C/T polymorphism

时间窗: 1 days

* Genetic polymorphism in the MMP9 coding region 1562C\>T was screened following the polymerase chain reaction and restriction fragment length polymorphism (RFLP-PCR). * The frequency distributions of different MMP9-1562 C/T genotypes and allele were investigated. * The relationship between the polymorphism of the MMP-9 gene and the severity of PU was analyzed.

Diabetes mellitus

时间窗: one hour

- Plasma levels glucose in mmol/l was measured by standard enzymatic methods using reagents in a fully automated analyzer Cx5 Pro-Bechman Coulter-Fuller-Ton

Inflammatory parameter

时间窗: one hour

- C-reactive protein (CRP), in mg/l, was measured using immunoturbidimetric methods (COBAS INTEGRA 400 Roche).

Markers of nutritional status

时间窗: one hour

* albumin (chronic marker) and prealbumin (early marker) were measured, in g/l, using the dry chemistry method (BN prospec, siemens). * Protide in g/l was measured by standard enzymatic methods using reagents in a fully automated analyzer (CX9-BECKMANN).

Genotyping of TNF- α G238A

时间窗: 1 days

* TNF-α G238A promoter polymorphism were determined by the RFLP-PCR method. * The genotypic and allelic frequencies of -238G/A were calculated * This study investigated the association between TNF-α-238G\>A and Pressure ulcer in Tunisian population.

Genotyping of TNF- α G308A

时间窗: 1 days

* The genotypic analysis of the TNF-α G308A polymorphism was performed using Allele-specific PCR (AS-PCR) amplification. * In this study, we have analyzed the TNF-α gene promoter -308G/A polymorphism in Tunisian patients with PU to evaluate the contribution of this SNP in genetic susceptibility to PU.