Microgravity Effects on Co-cultured Vascular Cells Types
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 2
- 试验地点
- 1
- 主要终点
- Differentiation (Colony Forming) Kinetics of normal vs microgravity cultured stem cells
研究概览
简要总结
The goal of this study is to assess the affect of ground based microgravity on co-cultured vascular cell types. Our goal is to better understand the cross-talk between vascular cell types grown under conditions such as microgravity as is seen in space. In this pilot study, the investigator proposes to assess changes in cell phenotype and function in microgravity as compared to normal gravity.
详细描述
This study builds upon previously reported space flight data in GenLab, as well as our own published data whereby the investigator evaluate the effect of microgravity on the differentiation and functional potential of circulating stem cells. The goal of this work is to leverage the powerful genetic analyses done in the SPHINX study and look at the consequences these changes have on cell-cell communications essential for vascular homeostasis, for a possible correlation to cardiovascular deconditioning. The application of this work is targeted at possible "cell based" countermeasures to address the negative effects of microgravity on the vasculature.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 21 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Self-reported healthy individuals
- •Willingness to have 50mls of whole blood collected
排除标准
- •Unwillingness to have blood used in stem cell research
结局指标
主要结局
Differentiation (Colony Forming) Kinetics of normal vs microgravity cultured stem cells
时间窗: Within 4 weeks of collecting cells
The study team are interesting in the effect of co-cultured Endothelial cells on the kinetics of stem cell colony formation (i.e. differentiation). As compared to normal gravity samples, the colonies that form while in co-culture with ECs will be counted and monitored. This gives a measure of the differentiation potential of these cells.
次要结局
- Gene and Protein expression of normal vs microgravity cultured stem cells(through study completion, up to 2 years from the start of the study)
- Anti-thrombogenic function of normal vs microgravity cultured stem cells(through study completion, up to 2 years from the start of the study)