Inhibiting Histone Deacetylase: Toward Eradication of HIV
试验速览
- 阶段
- 1 期
- 状态
- 终止
- 入组人数
- 14
- 试验地点
- 1
- 主要终点
- Frequencies of replication-competent HIV detected in resting CD4 cells
研究概览
简要总结
A histone deacetylase (HDAC) inhibitor is a class of drug that interferes with the function of HDAC, an enzyme that hides HIV within inactive CD4 cells. These drugs are normally used to treat seizures and other nervous system problems but have been found to work against HIV. The purpose of this study is to investigate the efficacy of valproic acid (VPA), an HDAC inhibitor, in treating HIV infected adults using anti-HIV drugs.
详细描述
VPA is a type of medication normally used to treat seizures and other nervous system problems. It has been found that VPA works against HIV by releasing the virus from resting CD4 cells, allowing other anti-HIV medications to attack it. The purpose of this study is to assess the efficacy of VPA when used in HIV infected participants using highly active antiretroviral therapy (HAART). The expected duration of participation for individually enrolled participants will depend on which study group the participant is placed in but may range from approximately 24 to 144 weeks.
An initial screening visit will occur about 30 to 120 days prior to study entry. A physical exam, medical and medication history assessment, and blood and urine collection will occur at screening. Leukapheresis and genital secretion collection will occur once 30 to 120 days prior to study entry, separate from the initial screening.
In Step 1, all participants will receive VPA while continuing their current HAART. Doses of VPA will vary by participant. Study visits will occur on Days 0 and 3 and Weeks 1, 2, 4, 8, 12, 16, and 24. A physical exam and blood and urine collection will occur at most visits. Leukapheresis and genital secretion collection will occur at study entry and Weeks 12 and 16. After 24 weeks, participants will enter Step 2. Those participants not responding to VPA in Step 1 will enroll in Step 2A. Participants responding to VPA in Step 1 will enroll in Step 2B.
In Step 2A, participants will discontinue VPA and will receive intensified therapy (enfuvirtide) administered for 24 weeks twice daily. Study visits will occur at Weeks 25, 28, 32, 36, 40, and 48. A physical exam and blood and urine collection will occur at all visits. Leukapheresis will occur at Weeks 36 and 40. Participants who do not respond to intensified therapy in Step 2A will enroll in Step 3A. Participants who respond to intensified therapy in Step 2A will enroll in Step 3B.
In Step 2B, participants will continue to receive VPA for up to 96 weeks. Study visits will occur every 8 weeks until Week 120. A physical exam and blood and urine collection will occur at each visit. Leukapheresis will occur once between Week 72 and Week 120.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •HIV-1 infected
- •Adherent to current HAART regimen
- •Adequate vascular access for leukapheresis
- •Receiving HAART, defined as at least two nucleoside reverse transcriptase inhibitors plus at least one protease inhibitor or non-nucleoside reverse transcriptase inhibitor, without changes to the regimen within 24 weeks of study entry
- •Viral load more than 50 copies/ml on two consecutive occasions for more than 6 months, and less than 200 copies/ml on occasion for more than 6 months prior to study entry
- •CD4 count more than 300 cells/mm3
- •Willing and able to comply with all study requirements
- •Willing to use acceptable forms of contraception
排除标准
- •Currently receiving zidovudine or enfuvirtide
- •Require certain medications known to interact with valproate (e.g., lamotrigine; barbiturates; carbamazepine; prescription dosages of salicylates, hydantoins, felbamate, and clonazepam)
- •Any medical, psychiatric, or job-related responsibility that would interfere with the study. More information about this criterion can be found in the protocol.
- •Contraindications to taking VPA (e.g., pregnancy, bleeding disorders, history of pancreatitis, history of hepatitis)
- •Receiving interferon, other immunomodulators, or other experimental medications
- •Abnormal liver enzyme tests
- •Hepatitis B virus infected
- •Symptoms of hepatic decompensation
- •Blood transfusions or hematopoietic growth factors within 90 days prior to study entry
- •Systemic cytotoxic chemotherapy, investigational agents, or immunomodulators within 90 days prior to study entry
- •Current drug or alcohol abuse that, in the opinion of the site investigator, would interfere with the study
- •Serious illness requiring systemic treatment or hospitalization within 90 days prior to study entry
- •Treatment for a current AIDS-defining opportunistic infection within 90 days prior to screening
- •Involuntarily incarcerated for treatment of either a psychiatric illness or physical illness (e.g., infectious disease)
- •Pregnancy or breastfeeding
研究组 & 干预措施
2A
Discontinuation of VPA and enfuvirtide administered for 24 weeks. As of 05/20/08 this step was discontinued.
干预措施: Enfuvirtide (Drug)
2B
Continuation of VPA for up to 96 weeks. As of 05/20/08 this step was discontinued.
干预措施: Valproic acid (Drug)
3A
VPA may be added to enfuvirtide for 16 weeks. VPA and enfuvirtide will be continued for up to 96 weeks in responders, and the study will be discontinued in nonresponders.
干预措施: Enfuvirtide (Drug)
3A
VPA may be added to enfuvirtide for 16 weeks. VPA and enfuvirtide will be continued for up to 96 weeks in responders, and the study will be discontinued in nonresponders.
干预措施: Valproic acid (Drug)
3B
Enfuvirtide may be continued for up to 96 weeks. As of 05/20/08 this step was discontinued.
干预措施: Enfuvirtide (Drug)
结局指标
主要结局
Frequencies of replication-competent HIV detected in resting CD4 cells
时间窗: At pre-entry and Week 0 to Weeks 12 and 16
次要结局
- Change in integrated proviral genomes(Throughout study)
- Genital tract proviral DNA and viral load(Throughout study)
- Detectable viral load during VPA therapy and if it remains undetectable after VPA is discontinued(Throughout study)
- HIV-specific antibody changes and CTL responses(From Week 0 to 16)
- Change in replication-competent HIV detected in resting CD4 cells in study participants after intensified HAART, and after extended VPA therapy with or without intensified HAART(Throughout study)
- Changes in viral load after intensification of HAART with or without VPA(Throughout study)