The Effects of Diesel Exhaust Inhalation on Vascular Function - the Role of Endothelin
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 1
- 主要终点
- Change in forearm blood flow following infusion of endothelin-1 or the endothelin receptor antagonists BQ-123 & BQ-788
研究概览
简要总结
The purpose of this study is to identify the role of endothelin 1 (a natural vasoconstrictor) in the adverse vascular effects demonstrated after exposure to air pollution
详细描述
Air pollution is a major problem, and can be linked to around 5% of all deaths worldwide each year. There are strong associations between air pollution exposure and heart disease but we do not yet understand how these harmful effects are mediated. Understanding this mechanism is likely to have a major impact on the way we treat patients with heart disease and have the potential to shape future environmental health policy. The upregulation of Endothelin-1 provides a plausible mechanism for these harmful effects, and we plan to investigate this in more depth in the human forearm following exposure to diesel exhaust.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy male volunteers
排除标准
- •Use of regular medication
- •Cigarette smoking
- •Significant occupational exposure to air pollution
- •Intercurrent illness
研究组 & 干预措施
1
1 hour exposure to filtered air, followed by forearm venous occlusion plethysmography at 2 hours after the exposure and infusion of endothelin-1 (5pmol/min)
干预措施: Endothelin-1 (Drug)
2
1 hour exposure to filtered air, followed by forearm venous occlusion plethysmography at 2 hours after the exposure and infusion of endothelin receptor antagonists BQ-123 and BQ-788
干预措施: BQ-123 and BQ-788 (Drug)
3
1 hour exposure to dilute diesel exhaust (300mcg/m3), followed by forearm venous occlusion plethysmography at 2 hours after the exposure and infusion of endothelin-1 (5pmol/min)
干预措施: Endothelin-1 (Drug)
4
1 hour exposure to dilute diesel exhaust (300mcg/m3), followed by forearm venous occlusion plethysmography at 2 hours after the exposure and infusion of endothelin receptor antagonists BQ-123 and BQ-788
干预措施: BQ-123 and BQ-788 (Drug)
结局指标
主要结局
Change in forearm blood flow following infusion of endothelin-1 or the endothelin receptor antagonists BQ-123 & BQ-788
时间窗: 2 hours after exposure
次要结局
- 24 hour mean blood pressure (ambulatory monitoring)(24 hours following the exposure)
- Systemic endothelin-1 and big endothelin-1 concentrations(At baseline, and immediately, 1, 2, 3, 4, 6 and 24 hours after exposure)
- Changes in arterial stiffness (pulse-wave velocity)(During and for the 1 hour after the exposure)