Assessment of the impact of Hibiscus Extract as Probiotics
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- 1. To evaluate the effect of Hibiscus sabdariffa extract on the growth and viability of Lactobacillus acidophilus by comparing colony-forming units (CFU/mL) in media with and without Hibiscus supplementation
研究概览
简要总结
This in-vitro laboratory study aims to evaluate the effect of Hibiscus sabdariffa extract on the growth and survival of Lactobacillus acidophilus. The probiotic strain will be cultured in De Man Rogosa and Sharpe media with and without Hibiscus extract supplementation. The growth will be assessed by colony-forming unit counts using the spread plate method. Observations will be recorded on Day 0, 15, 30, 45, and 60. The study seeks to identify the optimal concentration of Hibiscus extract that supports probiotic viability. Results will help determine the potential of Hibiscus as a functional ingredient in probiotic formulations to enhance gut health.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 盲法
- None
入排标准
- 年龄范围
- 18.00 Year(s) 至 40.00 Year(s)（—）
- 性别
- All
入选标准
- •Healthy individuals aged 18-40 years.
- •No chronic gastrointestinal diseases.
- •No history of antibiotic use in the past 4 weeks.
排除标准
- •Pregnant or lactating woman.
- •Individuals with known sensitivity to Hibiscus.
- •Those with recent gastrointestinal disturbances or treatments that can skew the results.
结局指标
主要结局
1. To evaluate the effect of Hibiscus sabdariffa extract on the growth and viability of Lactobacillus acidophilus by comparing colony-forming units (CFU/mL) in media with and without Hibiscus supplementation
时间窗: 2 months
次要结局
- 1. To determine the optimal concentration of Hibiscus extract that supports the highest growth and survival of Lactobacillus acidophilus under in vitro conditions(2 months)
研究者
Anjali Singh
Dr. BMN College of Home Science