Effects of Egg White Supplementation on Body Composition, Physical Performance, and Amino Acid Profile of Individuals Undergoing High-intensity Functional Training
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- Lean and fat mass pre-supplementation
研究概览
简要总结
High-intensity functional training (HIFT), a new version of high-intensity interval training, has gained interest in recent years. HIFT is based on the CrossFit training template and includes multijoint movement patterns via both endurance and strengthening exercises. Research has shown positive effects of HIFT on body composition, cardiorespiratory fitness, and muscle performance of young individuals. The effectiveness of HIFT in higher ages and its protein requirements are less well documented. Protein requirements have been widely investigated in resistance training, where it has been found that protein supplementation may have additive beneficial effects on muscle strength and lean body mass. However, there is a research gap regarding the adaptations to HIFT when combined with protein supplementation.
Thus, the aim of this research is to compare the effects of egg white supplementation, whey protein supplementation (as a positive comparator), and maltodextrin (a carbohydrate as placebo) on body composition, physical performance, and plasma amino acid profile in young and middle-aged trained individuals of both sexes who perform HIFT. Participants will take all three supplements for 6 weeks each, with 2 weeks of washout (no supplementation) in between, in random and counterbalanced order. Researchers will not know the supplementation status of the participants. Participants will receive 0.6 g of protein or placebo per kg body weight daily on top of isoenergetic dietary plans to avoid differences in energy intake that might compromise the validity of the study. The dietary plans will be individualized and will provided 1.0 g protein/kg body weight/day. The HIFT protocol will include multimodal patterns of movement, combining endurance and strengthening exercises with the use of equipment such as Total Resistance eXercise (TRX), Bosu, kettlebells, and barbells.
Participants will undergo measurements of muscle strength, muscle endurance, aerobic capacity, and body composition at the beginning and end of the study, as well as during the two washout periods. Also, plasma amino acids, hematology, biochemistry, and hormones will be measured. Comparison of all these outcome measures between supplements will reveal whether protein supplementation is useful in HIFT.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Other
- 盲法
- Triple (Care Provider, Investigator, Outcomes Assessor)
盲法说明
Participants are instructed not to disclose the supplement they are taking to their trainers, investigator, or outcome assessor.
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Regular training (mixed endurance and resistance training 3 to 6 times a week, 50 min each session, for the past 4 months), as assessed by gym records or questionnaires
- •Clearance from a pathologist or cardiologist to perform maximal exercise
- •Mixed isoenergetic diet for the past 4 months
排除标准
- •Smoking (even one cigarette or nicotine-containing device over the past 6 months)
- •Any injuries to the musculoskeletal system that could interfere with the execution of training
- •Chronic disease
- •Egg allergy
- •Milk allergy
- •Pregnancy, lactation, or planning a pregnancy within the duration of the study
- •Regular use of prescription medicine or supplements that might affect muscle function or recovery over the past month
- •Intermittent or religious fasting
- •Any vegetarian, ketogenic or protein diet
结局指标
主要结局
Lean and fat mass pre-supplementation
时间窗: Within 2 weeks before the beginning of supplementation.
Lean and fat mass of the whole body and its parts (trunk, legs and arms) will be assessed by dual X-ray absorptiometry.
Lean and fat mass post-supplementation
时间窗: Within 2 weeks after the end of supplementation.
Lean and fat mass of the whole body and its parts (trunk, legs and arms) will be assessed by dual X-ray absorptiometry.
Muscle endurance pre-supplementation
时间窗: Within 2 weeks before the beginning of supplementation.
Muscle endurance will be assessed by performing multiple sit-ups, knee flexions and knee extensions.
Maximal dynamic strength of shoulder muscles post-supplementation
时间窗: Within 2 weeks after the end of supplementation.
Maximal dynamic strength of shoulder muscles will be assessed by measurement of one-repetition maximum.
Force-velocity relationship of knee flexors and extensors pre-supplementation
时间窗: Within 2 weeks before the beginning of supplementation.
Force-velocity relationship of knee flexors and extensors will be assessed in isokinetic dynamometer.
Plasma amino acid profile pre-supplementation
时间窗: Within 4 days before the beginning of supplementation.
Plasma amino acid profile (that is, the concentration of each individual amino acid) will be determined by liquid chromatography - mass spectrometry.
Aerobic fitness pre-supplementation
时间窗: Within 2 weeks before the beginning of supplementation.
Aerobic fitness will be assessed by measurement of maximal oxygen uptake through a maximal graded exercise test on treadmill.
Maximal dynamic strength of shoulder muscles pre-supplementation
时间窗: Within 2 weeks before the beginning of supplementation.
Maximal dynamic strength of shoulder muscles will be assessed by measurement of one-repetition maximum.
Muscle endurance post-supplementation
时间窗: Within 2 weeks after the end of supplementation.
Muscle endurance will be assessed by performing multiple sit-ups, knee flexions and knee extensions.
Force-velocity relationship of knee flexors and extensors post-supplementation
时间窗: Within 2 weeks after the end of supplementation.
Force-velocity relationship of knee flexors and extensors will be assessed in isokinetic dynamometer.
Plasma amino acid profile post-supplementation
时间窗: Within 4 days after the end of supplementation.
Plasma amino acid profile (that is, the concentration of each individual amino acid) will be determined by liquid chromatography - mass spectrometry.
Aerobic fitness post-supplementation
时间窗: Within 2 weeks after the end of supplementation.
Aerobic fitness will be assessed by measurement of maximal oxygen uptake through a maximal graded exercise test on treadmill.
次要结局
- Plasma enzymes post-supplementation(Within 4 days after the end of supplementation.)
- Biochemical analyses post-supplementation(Within 4 days after the end of supplementation.)
- Internal load of exercise pre-supplementation(Within the first week of supplementation.)
- Internal load of exercise post-supplementation(Within the last week of supplementation (week 6).)
- Hormonal analyses pre-supplementation(Within 4 days before the end of supplementation.)
- Full blood count pre-supplementation(Within 4 days before the beginning of supplementation)
- Hormonal analyses post-supplementation(Within 4 days after the end of supplementation.)
- Resting metabolic rate pre-supplementation(Within 2 weeks before the beginning of supplementation.)
- Full blood count post-supplementation(Within 4 days after the end of supplementation.)
- Maximal grip strength pre-supplementation(Within 2 weeks before the beginning of supplementation)
- Maximal grip strength post-supplementation(Within 2 weeks after the end of supplementation.)
- Biochemical analyses pre-supplementation(Within 4 days before the beginning of supplementation.)
- Resting metabolic rate post-supplementation(Within 2 weeks after the end of supplementation.)
- Plasma enzymes pre-supplementation(Within 4 days before the beginning of supplementation.)
- Force-velocity relationship of shoulder muscles pre-supplementation(Within 2 weeks before the beginning of supplementation.)
- Force-velocity relationship of shoulder muscles post-supplementation(Within 2 weeks after the end of supplementation.)