Metabolism in Adipose Tissue Followed Following Sprint Exercise
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 18
- 试验地点
- 2
- 主要终点
- Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
研究概览
简要总结
Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.
详细描述
PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.
METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.
SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Physical active
排除标准
- •On medication
- •No infection
- •No asthma
结局指标
主要结局
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
时间窗: After 30-s repeated sprint exercise
Stomach fat biopsies and arterial and stomach vein blood are obtained
次要结局
- Changes in blood flow in adipose tissue(After 30-s repeated sprint exercise)
- Changes in different metabolites(After 30-s repeated sprint exercise)
研究者
Mona Esbjörnsson
Associate Professor
Karolinska Institutet