Mitochondrial Energy Metabolism in Obese Women Undergoing Concurrent Physical Training
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 14
- 试验地点
- 2
- 主要终点
- Changes inflammatory cytokines
研究概览
简要总结
Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:
- The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;
- The unbalance is therapeutically modified through physical training ;
- Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.
This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.
详细描述
Specific objectives:
Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot.
The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample
排除标准
- •Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.
研究组 & 干预措施
Physical Training
There was concurrent physical training intervention: strength and aerobic exercises in the same session.
Duration: 2 weeks of adaptation and learning to exercise, 8 weeks of physical training.
Frequency: 3 times per week Duration: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.
干预措施: Physical Training (Other)
结局指标
主要结局
Changes inflammatory cytokines
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Changes Physical Performance
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Based on the Shuttle Walking Test adaptation.
Changes Telomere length
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell. The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).
Changes in fatty acids
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Changes in total cholesterol
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Changes in Determination of Lactate
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.
Changes Nitrogen Balance
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.
Changes Indirect calorimetry
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).
Changes oxidative stress
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Changes Food intake
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.
Changes Body weight
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Body weight was measured by digital balance before and after the intervention
Changes Body composition
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
The change in body composition through deuterium oxide was evaluated.
Changes White adipose tissue biopsy
时间窗: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).
次要结局
未报告次要终点
研究者
Julio Sergio Marchini
Principal Investigator
University of Sao Paulo