CD180 Overexpression in Follicular Lymphoma Is Restricted to the Lymph Node Compartment
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 184
- 主要终点
- Immunophenotype Analysis by Flow Cytometry of peripheral blood samples at inclusion
研究概览
简要总结
Background:Altered Toll-like receptor (TLR) expression levels and/or mutations in its signaling pathway (such as MyD88 mutation) contribute to the pathogenesis of lymphoproliferative disorders (LPD). CD180 is an orphan member of the TLR family that modulates the signaling of several TLRs, but only limited studies have evaluated its expression by flow cytometry (FCM) in LPD.
Methods: Using a multiparameter FCM approach, biologists have assessed CD180 mean fluorescence intensity (MFI) in lymph nodes (LNs) and peripheral blood (PB) samples obtained from patients with follicular lymphoma (FL; LN/PB, n=44/n=15), chronic lymphocytic leukemia (CLL, n=26/n=21), mantle cell lymphoma (MCL, n=13/n=17), and marginal zone lymphoma (MZL, n=16/n=12). Specimens from non-tumoral PB and LN (n=8/n=12) were used as controls.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Samples from patients with:
- •LF: Follicular lymphoma
- •MCL: Mantle cell lymphoma
- •CLL/SLL: chronic lymphocytic leukemia/small lymphocytic leukemia
- •MZL : splenic marginal zone
- •Clt :control B-cells
排除标准
- •Patient <18 years old
结局指标
主要结局
Immunophenotype Analysis by Flow Cytometry of peripheral blood samples at inclusion
时间窗: At inclusion, day 0.
Using a multiparameter FCM approach, biologists assessed the CD180 expression by our routine antigen panel in LN suspensions as well as in PB samples. Samples were analyzed on a BD FACSCanto II cytometer (BD FACS DIVA software; Becton Dickinson, San Jose, CA).
Immunophenotype Analysis by Flow Cytometry of lymph nodes samples at inclusion
时间窗: At inclusion, day 0.
Using a multiparameter FCM approach, biologists assessed the CD180 expression by our routine antigen panel in LN suspensions as well as in PB samples. Samples were analyzed on a BD FACSCanto II cytometer (BD FACS DIVA software; Becton Dickinson, San Jose, CA).
次要结局
未报告次要终点