Investigation of The Efficiency of Pulsed Electromagnetic Field Treatment and Stretching Exercise In Experimental Skeletal Muscle Injury Model
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 64
- 试验地点
- 1
- 主要终点
- Mononuclear cell infiltration, Atrophy and necrosis, Pyknotic nucleus assesment at the end of two weeks
研究概览
简要总结
In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.
详细描述
Purpose: The aim of this study was to evaluate the efficacy of stretching exercise and the pulsed electromagnetic field (PEMF) method in the treatment of muscle injury (INJ).
Methods: In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 10 Months 至 11 Months（Child）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Healthy young wistar albino rats
排除标准
- •not having any injury
研究组 & 干预措施
Pulsed Electromagnetic Field
干预措施: Exercise (Other)
Control
Stretching Exercise
干预措施: Pulsed Electromagnetic Field Therapy (Device)
Pulsed Electromagnetic Field and Stretching Exercise
干预措施: Exercise (Other)
Pulsed Electromagnetic Field and Stretching Exercise
干预措施: Pulsed Electromagnetic Field Therapy (Device)
结局指标
主要结局
Mononuclear cell infiltration, Atrophy and necrosis, Pyknotic nucleus assesment at the end of two weeks
时间窗: two week
hematoxylin-eosin staining
TGF-β1, eNOS assesment at the end of two weeks
时间窗: two week
Quantitative Reverse-Transcriptase Polymerase Chain Reaction (RT-qPCR)
VEGF and FGF assesment at the end of two weeks
时间窗: two week
immunohischemical staining
次要结局
未报告次要终点
研究者
Mesut ERGAN
Assistant Professor
Suleyman Demirel University