The Blastocyst Development and Clinical Outcome in Single Step Medium With or Without Renewal on Day 3 in a Time-lapse Incubator
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 200
- 试验地点
- 1
- 主要终点
- Clinical pregnancy rate
研究概览
简要总结
Culture media used to support development of zygotes to the blastocyst stage is based on two basic philosophies: a single step medium "let-the-embryo-choose" or a sequential medium "back-to-nature".
For single step media, there are two types of protocol can be used for the culture of human preimplantation embryos:
- Interrupted single medium culture, in which the medium is renewed on the Day 3 of embryo culture
- Uninterrupted single embryo culture, in which the same single medium is used throughout the 5-6 days of culture with no replenishment or replacement on Day 3.
Investigators aim to compare the blastocyst development and clinical outcome between the two types of protocols of a single medium (with or without renewal on Day 3)
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 盲法
- Double (Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 19 Years 至 39 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •female partners were under the age of 39 years
- •Intracytoplasmic sperm injection (ICSI) was performed using fresh ejaculates
排除标准
- •Day 3 embryo transfer cycles
研究组 & 干预措施
Uninterrupted single embryo culture
The same single medium is used throughout the 5-6 days of culture with no replenishment on day 3.
Interrupted single medium culture
The single step medium is renewed on Day 3 of embryo culture.
干预措施: Culture media renwal (Procedure)
结局指标
主要结局
Clinical pregnancy rate
时间窗: 4 weeks post embryo transfer
diagnosed by the presence of a heartbeat in the gestational sac visualized by ultrasound
次要结局
- Blastocyst formation rate(5-6 days post intracytoplasmic sperm injection)
- Implantation rate(4-6 weeks post embryo transfer)
研究者
Yasmin
Senior clinical embryologist
Dar AlMaraa Center