Searching for the New Mechanisms That Activate Brow Fat Tissue
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 主要终点
- The potential associations between BAT activity and dietary intake
研究概览
简要总结
The general purpose of the study was to provide new information about the role of macronutrients intake, metabolomics, proteomics and microRNA on BAT activation.
The invesigator evaluated BAT activity and whole body energy consumption under cold stimulation in two gruops of healthy males aged 21-43 years old with normal BMI ( 19-25kg/m2) and in overweight/obese subjects.
详细描述
In detail, the main objectives of this study was to measure the influence of biological molecules of such fields: genomics, transcriptomics metabolomics and protemics and its relationship on BAT activation. The investigator also evaluated the role of diet, macronutirnets intake and polyunsaturated fatty acids on BAT activity.
Study design:
- SCREENING VISIT:
- obtaining written informed consent from all subjects
- physical examination
- body composition analysis ( DXA + bioindepedance method inbody 720)
Laboratory analyses:
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 43 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Inclusion criteria to the group 1 (healthy males) N=20:
- •BMI <25 kg/m2;
- •RR<140/90mmHg;
- •age 20-30/40 years
- •fasting glycemia <100mg/dl; 2h-OGTT <140mg/dl;
- •no chronic disease
- •Inclusion criteria to the group 2 ( overweight/obese males) N=20:
- •Blood pressure < 140/90 mmHg (well controlled by inh ACE; no beta-blockers)
- •No chronic disease
- •BMI > 25 <35kg/m²
- •fasting glycemia <100mg/dl; 2h-OGTT <140mg/dl;
排除标准
- •Any drug use: beta-blokers, statins, glucocorticoids drugs, hypertension drugs;
- •hypertension;
- •hormonal disorders;
- •Inflammation process
- •Claustrophobia
结局指标
主要结局
The potential associations between BAT activity and dietary intake
时间窗: 2016-2018
The relationship between BAT activity and dietary intake will be analyzed
The potential associations between BAT activity and metabolite profile
时间窗: the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure
The potential molecules will be identified by metabolomics approach and will be analyzed dependently on the brown tissue activity level
The potential associations between BAT activity and proteins profile
时间窗: the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure
The potential molecules will be identified by proteomics approach and will be analyzed dependently on the brown tissue activity level
The potential associations between BAT activity and transcriptome profile
时间窗: the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure
The potential molecules will be identified by transriptomics approach and will be analyzed dependently on the brown tissue activity level
次要结局
- The potential associations between BAT activity and irisin concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and atrial natiuretic peptide concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and fT3 concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and Interleukin-6 concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and FFA ( free fatty acids) concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and energy expenditure(at the baseline ( -30 min to 0 min) and every 30 min until 120 min of cold exposure)
- The potential associations between BAT activity and glucose concentrations(he baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and TSH concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and substrates utilization(at the baseline ( -30 min to 0 min) and every 30 min until 120 min of cold exposure.)
- The potential associations between BAT activity and insulin concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and brain natiuretic peptide concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and plasma nonesterified fatty acid concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and fT4 concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposu)