Diagnostic Interest of Leukocyte Immunophenotyping Using Multiparameter 8-color Flow Cytometry in Heart Surgery
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Expression of the marker CD64, CD10 and CD16
研究概览
简要总结
In septic patients, Septiflux1 study identified immature granulocytes which may have a diagnostic and prognostic interest. However, with these preliminary results it is not possible to make a difference between what is linked to the infection and what is a consequence of the inflammation which goes along with the septic process. Using flow cytometry, the study of leukocytes in a pure inflammatory model such as cardiopulmonary bypass in heart surgery could make possible to test the diagnostic interest of the immature granulocytes but also to attribute a prognostic value for the occurrence of post-operative infections.
详细描述
Expression of CD10, CD16, CD24, CD64 on granulocytes, of CD14 and CD16 on monocytes and of CD3 on T lymphocytes.
Flow cytometers will all be calibrated the same way using common reagents (known fluorescent calibration beads). Moreover, CD45 and CD3 level of expression on lymphocytes will help to check that the collection of data is standardized between the different centers.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patient older than 18 years old
- •Patient undergoing planned heart surgery with cardiopulmonary bypass
排除标准
- •Pregnancy,
- •progressive solid cancer,
- •HIV infection,
- •history of blood or inflammatory disease,
- •long-term immunosuppressive treatment,
- •urgent surgical intervention,
- •documented preoperative infection
结局指标
主要结局
Expression of the marker CD64, CD10 and CD16
时间窗: 1 hour
Evolution rate of CD64, CD10 and CD16 in the immediate postoperative cardiac surgery
次要结局
- Early onset of infectious complications(7 days)