NCT06205771Enrolling By Invitation不适用
The Role and Mechanism of GPER Hippo Pathway in Regulating Uterine Arterial Smooth Muscle CBS/H2S in the Pathogenesis of Preeclampsia
Hao Feng1 个研究点 分布在 1 个国家目标入组 54 人开始时间: 2024年4月10日最近更新:
适应症
试验速览
- 阶段
- 不适用
- 状态
- Enrolling By Invitation
- 发起方
- 入组人数
- 54
- 试验地点
- 1
- 主要终点
- H2S content in uterine arterial smooth muscle circulation
研究概览
简要总结
The goal of this clinical trial is to learn about in health conditions. The main questions it aims to answer are:
- The pathological significance of GPER in uterine artery dilation in preeclampsia
- The Mechanism of GPER Hippo Pathway Regulating CBS/H2S in Human Uterine Artery Smooth Muscle Cells (hUASMC) This project intends to use GPER interfering RNA, YAP1 interfering RNA, in vivo perfusion experiments of human uterine artery tissue, and single cell patch clamp technology to study hypotheses under physiological/pathological pregnancy conditions at the tissue, cellular, and molecular levels, revealing a novel signal transduction pathway of estrogen stimulating vasodilation, providing new ideas for studying the mechanism of uterine artery blood flow regulation. This research result will provide new targets for intervention and treatment of diseases such as fetal intrauterine growth retardation and preeclampsia.
详细描述
- Measure the content of H2S in uterine arterial smooth muscle and circulation of normal pregnancy and PE pregnant women, and analyze the relationship between the content of H2S in uterine arterial smooth muscle and circulation of PE pregnant women and PE.
- Knock down the GPER and overexpression of GPER in HTR-8 cells, and detect the expression of GPER, CBS, and Hippo pathway related proteins using Western blot and qRT-PCR to verify the efficiency of knockdown and overexpression. Use different concentrations of H2S donor (GYY4137) to interfere with HTR-8 cells after treatment, and use RT-PCR and Western blot to detect the mRNA and protein expression of downstream molecules YAP and TAZ in Hippo pathway in cells, respectively.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- — 至 40 Years（Child, Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Full term pregnancy with clinical diagnosis of preeclampsia and normal pregnancy pregnant woman
- •Pregnant women undergoing lower segment cesarean section for delivery
排除标准
- •Combined with chronic hypertension, kidney disease, intrahepatic cholestasis, etc Other basic diseases;
- •Multiple pregnancy
- •Pregnant women undergoing vaginal delivery
- •Normal pregnant women who are<37 weeks or>40 weeks pregnant
结局指标
主要结局
H2S content in uterine arterial smooth muscle circulation
时间窗: 8:00 am
H2S content in circulation
次要结局
未报告次要终点
研究者
Hao Feng
professor
Qianfoshan Hospital
研究点 (1)
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