Ethnic Differences in Iron Absorption
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 515
- 试验地点
- 2
- 主要终点
- The concentrations of iron and micronutrient status indicators
研究概览
简要总结
This study aims to; 1) investigate population differences in iron absorption between East Asians and Northern Europeans; 2) assess population differences in hormonal and biochemical determinants of Fe absorption between East Asians and Northern Europeans; and 3) to investigate genetic contributions to Fe absorption, Fe status and Fe regulatory hormones between East Asians and Northern Europeans.
详细描述
Detailed description:
This study will utilize a multidisciplinary approach to identify genetic variation in genes that control iron utilization in order to shed light on the genetic basis of population differences in iron status and disease susceptibility with a long-term goal of informing population-specific dietary iron intake recommendations to minimize the risk of chronic diseases. To evaluate iron utilization, we will employ an in vivo, functional approach using an oral stable iron isotope method. Each participant will have genetic ancestry and genotyping evaluated using the Illumina Global Diversity Array-8. Study participants (n=504, aged 18-50 y) will consume 57Fe (as ferrous sulfate) in the fasted state and will then ingest a standardized breakfast and lunch meal. Two weeks after iron dosing, a blood sample will be collected from each participant and the amount of 57Fe incorporated into red blood cells will be measured using magnetic sector thermal ionization mass spectrometry. This project will fundamentally advance our understanding of ethnic differences in nutrient metabolism and iron status. It will also provide information to assist with the long-term goal of reducing the public health burden of Fe-related diseases.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy adults
- •Age between 18- 50y
- •Non-smoking
- •Not taking vitamin or mineral supplements.
- •Females: premenopausal and not pregnant or lactating
- •No preexisting medical complications (such as eating disorders, hemoglobinopathies, malabsorption diseases, steroid use, substance abuse history, or taking medications known to influence iron homeostasis)
- •Body mass index (BMI) between 18 - 30 kg/m2.
排除标准
- •BMI <18 or > 30 kg/m2,
- •Age <18 y or > 50y,
- •Not of Northern European or East Asian ancestry
- •Pregnancy, lactating
- •Have gastrointestinal disorders/malabsorption diseases/hemoglobinopathies/dietary restrictions/steroid use/ medication use of medications known to impact iron status, iron utilization or inflammatory status
- •Take vitamin and mineral supplementations
结局指标
主要结局
The concentrations of iron and micronutrient status indicators
时间窗: baseline and two-weeks post dosing
The concentrations or serum folate, B12, hemoglobin, hematocrit, transferrin receptor, hepcidin, erythropoietin, erythroferrone, ferritin, interleukin-6, and c-reactive protein
Genetic ancestry and characterization of iron-related genotypes
时间窗: baseline
The DNA will be extracted from whole blood samples and each participant will have genetic ancestry and genotyping evaluated using the Illumina Global Diversity Array-8.
The percent of non-heme iron absorption
时间窗: 2-week
The percent of non-heme iron absorption will be determined by red blood cell iron incorporation of stable 57Fe
Habitual dietary information
时间窗: baseline
Habitual dietary information will be obtained from Diet History Questionnaire III .
Dietary information on the study day
时间窗: baseline
Detailed dietary information about all foods and beverages consumed on the study day will be obtained from the Automated Self-Administered 24-Hour Dietary Assessment Tool.
次要结局
未报告次要终点