Proliferation and Osteogenic Differentiation of Bone Marrow-derived Mesenchymal Stromal Cells From Osteoarthritic Versus Healthy Donors
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Global Gene expression profile of bone marrow-derived mesenchymal stromal cells from osteoarthritic versus healthy donors
研究概览
简要总结
Osteoarthritis (OA) is one of the most frequent musculoskeletal disorders and represents the main indication for total joint arthroplasty. Multipotent mesenchymal stromal cells (MSCs) can be easily isolated and culture expanded from bone marrow aspirates and provide an excellent source of progenitor cells for cell-based regeneration strategies due to their ex vivo differentiation and proliferation capacity. Although there are hints that MSCs derived from OA patients may exhibit altered function the role of MSCs with respect to disease development and progression of OA is not clearly understood to date. To assess whether advanced-stage OA affects MSCs' suitability for musculoskeletal regenerative therapy, in the present study, we compare proliferation and differentiation potential of MSCs from osteoarthritic versus healthy donors.
详细描述
Clinical data (range of motion of lower limb joints, sociodemographic score dataset, WOMAC score, EQ-5D score, Harris Hip Score, radiological evaluation (OA group only), routine laboratory parameters, and bone metabolism parameters)
Osteogenic, chondrogenic, and adipogenic differentiation is proofed qualitatively by cell staining
osteogenic, chondrogenic and adipogenic marker gene expression analysis using quantitative real-time RT-PCR
cell-specific alkaline phosphatase (ALP) activity assay
large-scale gene expression profile
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 50 Years 至 90 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •indication for hip THR
- •primary osteoarthritis of the hip
- •Patient's consent
排除标准
- •secondary osteoarthritis of the hip
- •Any malignancies
- •infectious disease
- •rheumatic disease
- •osteoporosis
- •Any additional serious disease complicating the participation in this study
结局指标
主要结局
Global Gene expression profile of bone marrow-derived mesenchymal stromal cells from osteoarthritic versus healthy donors
时间窗: after msc isolation
次要结局
- Flow cytometric analysis of cell surface antigens, Alkaline phosphatase activity, DNA content as measure for cellular proliferation(variable 2 days up to 21 days after msc cultivation)