Revitalization of Teeth With Necrotic Pulps and Open Apexes Using Platelet-Rich Plasma

Procedures Participants in this study must be in overall good health and not allergic to the materials used during this study treatment. If you agree to participate in this study, you will receive a screening visit to determine whether you will qualify for the study. At that time, your medical history will be reviewed, your blood pressure and pulse will be taken and you will be asked to fill out detailed medical and dental histories. In addition, you will receive an examination of your teeth and gums.

In the first appointment, after anesthetizing you with a local anesthetic similar to Novocain and application of a rubber dam to isolate your tooth, an opening will be made into the root canal(s). The canal(s) will be cleaned and medicated with antibiotics to get rid of the infection and sealed with a temporary filling material. Except for the use of antibiotics the rest of the procedures done in this appointment are similar to what the investigators do for the patients for need root canal treatment.

In the second appointment, after providing anesthesia with Novocain and application of rubber dam again, the temporary filling will be removed and the antibiotic will be removed using sterile water. The canal will be then dried. During this appointment, 20 ml of whole blood will be taken from your arm vein and will be used to prepare concentrated cells for injection into the prepared canal and sealed again with a temporary filling material. This part is different than what the investigators normally do for patients who need root canal treatment. For those patients the investigators fill the canals and seal the opening into the root canals with a permanent filling material.

You will return three to seven days later to the endodontic clinic and at that time, after rubber dam application, the temporary filling material will be removed and the tooth will be double sealed with permanent filling materials. This is an extra step that the investigators normally do not take for patients who need root canal treatment. A dental assistant or a dentist will call you to make an appointment at 3, 6, 12, 18, and 24 months following completion of your root canal treatment to check the vitality of the tooth using an ice stick and closure of the root end. Checking the process of healing in your tooth is similar to what the investigators routinely do for our patients who require root canal treatment.

Risks The study will require drawing blood from your arm. Veins and arteries vary in size from one person to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others. Other risks associated with having blood drawn are slight but may include bleeding, fainting or feeling light-headed, hematoma (blood accumulating under the skin), and infection (a slight risk any time the skin is broken). In rare instances, it may cause severe pain of infection. Depending upon the complications, your dentist may decide to remove the PRP completely out of the root canal and the use other procedures to treat your tooth. Should root canal failure occur, further dental treatment will be rendered at no charge to you.