Effect of Epstein-Barr Virus on Macrophage M2/M1 Migration and EphA2 Expression in Adverse Drug Reactions
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- 入组人数
- 27
- 试验地点
- 1
- 主要终点
- changes in the levels of monocytes, macrophages, M2/M1 ratio, and cytokines
研究概览
简要总结
This study aims to investigate the effect of EBV reactivation or EBV reactivation added with dexamethasone(DXM) in patients with adverse drug reactions(CDR) , through evaluating the levels on monocyte, macrophage M2/M1 and cytokines. To investigate whether expression of EBV receptors EphA2 could specifically influence on EBV activation in CDRs. We performed a prospective longitudinal study on the frequencies of Monocyte, Macrophage, M2/M1 and cytokines included IL-4,IL-13,TNF-α,IFN-γ,IFN-β, CXCL9 and CXCL10 after onset of MPE ,SJS/TEN and control groups. PBMCs collected from peripheral blood were cocultured with EBV or EBV with DXM. We next examined whether EBV or EBV with DXM could have a strong impact on the MOs, Mac, M2/M1 and cytokines and which cytokines could be crucial for the interaction between M2/M1 and EBV, by in vitro cocultures. Finally, EphA2 were detected to evaluate reactivation of EBV.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age and Gender: The study includes male and female patients aged 18 years and older.
- •a.Erythema Multiforme (MPE) Patients: Diagnosed based on clinical presentation and medical examination findings.b.Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis (SJS/TEN) Patients: Diagnosed according to the criteria set by the International Dermatology Society for SJS or TEN.c.Control Group: Healthy volunteers, without any history of chronic disease or acute infection, and not currently on any medications.
排除标准
- •Other Chronic Diseases: Patients with active heart, liver, kidney disease, or other systemic conditions.
- •Acute Infection: Patients who have had a viral or bacterial infection within the month prior to enrollment.
- •Immune Status: Patients who are immunocompromised or are receiving immunosuppressive therapy.
- •Allergy History: Patients with a known allergy to any drugs or agents used in the study.
- •Pregnancy or Lactation: Women who are currently pregnant or breastfeeding.
结局指标
主要结局
changes in the levels of monocytes, macrophages, M2/M1 ratio, and cytokines
时间窗: 24 hours and 48 hours
次要结局
未报告次要终点
研究者
Tang Junting
Director
First Affiliated Hospital of Kunming Medical University